8rw1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of a yeast 48S-AUC preinitiation complex in closed conformation== | |
| + | <StructureSection load='8rw1' size='340' side='right'caption='[[8rw1]], [[Resolution|resolution]] 3.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8rw1]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RW1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.35Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rw1 OCA], [https://pdbe.org/8rw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rw1 RCSB], [https://www.ebi.ac.uk/pdbsum/8rw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rw1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q6CKL3_KLULA Q6CKL3_KLULA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In eukaryotic translation initiation, the 48S preinitiation complex (PIC) scans the 5' untranslated region of mRNAs to search for the cognate start codon (AUG) with assistance from various eukaryotic initiation factors (eIFs). Cognate start codon recognition is precise, rejecting near-cognate codons with a single base difference. However, the structural basis of discrimination of near-cognate start codons was not known. We have captured multiple yeast 48S PICs with a near-cognate AUC codon at the P-site, revealing that the AUC codon induces instability in the codon-anticodon at the P-site, leading to a disordered N-terminal tail of eIF1A. Following eIF1 dissociation, the N-terminal domain of eIF5 fails to occupy the vacant eIF1 position, and eIF2beta becomes flexible. Consequently, 48S with an AUC codon is less favourable for initiation. Furthermore, we observe hitherto unreported metastable states of the eIF2-GTP-Met-tRNAMet ternary complex, where the eIF2beta helix-turn-helix domain may facilitate eIF5 association by preventing eIF1 rebinding to 48S PIC. Finally, a swivelled head conformation of 48S PIC appears crucial for discriminating incorrect and selection of the correct codon-anticodon pair during translation initiation. | ||
| - | + | Structural basis of AUC codon discrimination during translation initiation in yeast.,Villamayor-Belinchon L, Sharma P, Gordiyenko Y, Llacer JL, Hussain T Nucleic Acids Res. 2024 Aug 28:gkae737. doi: 10.1093/nar/gkae737. PMID:39193907<ref>PMID:39193907</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8rw1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Kluyveromyces lactis NRRL Y-1140]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hussain T]] | ||
| + | [[Category: Llacer JL]] | ||
| + | [[Category: Sharma P]] | ||
| + | [[Category: Villamayor-Belinchon L]] | ||
Current revision
Structure of a yeast 48S-AUC preinitiation complex in closed conformation
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