1q71

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[[Image:1q71.gif|left|200px]]
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{{Seed}}
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[[Image:1q71.png|left|200px]]
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{{STRUCTURE_1q71| PDB=1q71 | SCENE= }}
{{STRUCTURE_1q71| PDB=1q71 | SCENE= }}
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'''The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone'''
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===The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone===
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==Overview==
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Microcin J25 is a 21 amino acid bacterial peptide that has potent antibacterial activity against Gram-negative bacteria, resulting from its interaction with RNA polymerase. The peptide was previously proposed to have a head-to-tail cyclized peptide backbone and a tight globular structure (Blond, A., Peduzzi, J., Goulard, C., Chiuchiolo, M. J., Barthelemy, M., Prigent, Y., Salomon, R. A., Farias, R. N., Moreno, F. &amp; Rebuffat, S. Eur. J. Biochem. 1999, 259, 747-755). It exhibits remarkable thermal stability for a peptide of its size lacking disulfide bonds and in part this was previously proposed to derive from its macrocyclic structure. We show here that in fact the peptide does not have a head-to-tail cyclic structure but rather a side chain to backbone cyclization between Glu8 and the N-terminus. This creates an embedded ring that is threaded by the C-terminal tail of the molecule, forming a noose-like feature. The three-dimensional structure deduced from NMR data suggests that slippage of the noose is prevented by two aromatic residues flanking the embedded ring. Unthreading does not occur even when the molecule is enzymatically digested with thermolysin. The new structural interpretation fully accounts for previously reported NMR and biophysical data and is consistent with the remarkable stability of this potent antimicrobial peptide.
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The line below this paragraph, {{ABSTRACT_PUBMED_14531690}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14531690 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14531690}}
==About this Structure==
==About this Structure==
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1Q71 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA].
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1Q71 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA].
==Reference==
==Reference==
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[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Sidechain-to-backbone link]]
[[Category: Sidechain-to-backbone link]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:56:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:21:04 2008''

Revision as of 01:21, 28 July 2008

Image:1q71.png

Template:STRUCTURE 1q71

The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone

Template:ABSTRACT PUBMED 14531690

About this Structure

1Q71 is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone., Rosengren KJ, Clark RJ, Daly NL, Goransson U, Jones A, Craik DJ, J Am Chem Soc. 2003 Oct 15;125(41):12464-74. PMID:14531690

Page seeded by OCA on Mon Jul 28 04:21:04 2008

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