1q9h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1q9h.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1q9h.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1q9h| PDB=1q9h | SCENE= }}
{{STRUCTURE_1q9h| PDB=1q9h | SCENE= }}
-
'''3-Dimensional structure of native Cel7A from Talaromyces emersonii'''
+
===3-Dimensional structure of native Cel7A from Talaromyces emersonii===
-
==Overview==
+
<!--
-
The X-ray structure of native cellobiohydrolase IB (CBH IB) from the filamentous fungus Talaromyces emersonii, PDB 1Q9H, was solved to 2.4 A by molecular replacement. 1Q9H is a glycoprotein that consists of a large, single domain with dimensions of approximately 60 A x 40 A x 50 A and an overall beta-sandwich structure, the characteristic fold of Family 7 glycosyl hydrolases (GH7). It is the first structure of a native glycoprotein and cellulase from this thermophilic eukaryote. The long cellulose-binding tunnel seen in GH7 Cel7A from Trichoderma reesei is conserved in 1Q9H, as are the catalytic residues. As a result of deletions and other changes in loop regions, the binding and catalytic properties of T. emersonii 1Q9H are different. The gene (cel7) encoding CBH IB was isolated from T. emersonii and expressed heterologously with an N-terminal polyHis-tag, in Escherichia coli. The deduced amino acid sequence of cel7 is homologous to fungal cellobiohydrolases in GH7. The recombinant cellobiohydrolase was virtually inactive against methylumberiferyl-cellobioside and chloronitrophenyl-lactoside, but partial activity could be restored after refolding of the urea-denatured enzyme. Profiles of cel7 expression in T. emersonii, investigated by Northern blot analysis, revealed that expression is regulated at the transcriptional level. Putative regulatory element consensus sequences for cellulase transcription factors have been identified in the upstream region of the cel7 genomic sequence.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15560790}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15560790 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15560790}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Tuohy, M G.]]
[[Category: Tuohy, M G.]]
[[Category: Cellobiohydrolase i catalytic domain]]
[[Category: Cellobiohydrolase i catalytic domain]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:02:05 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:00:27 2008''

Revision as of 13:00, 28 July 2008

Image:1q9h.png

Template:STRUCTURE 1q9h

3-Dimensional structure of native Cel7A from Talaromyces emersonii

Template:ABSTRACT PUBMED 15560790

About this Structure

1Q9H is a Single protein structure of sequence from Talaromyces emersonii. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii., Grassick A, Murray PG, Thompson R, Collins CM, Byrnes L, Birrane G, Higgins TM, Tuohy MG, Eur J Biochem. 2004 Nov;271(22):4495-506. PMID:15560790

Page seeded by OCA on Mon Jul 28 16:00:27 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1q9h"
Personal tools