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| - | [[Image:1qcm.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1qcm| PDB=1qcm | SCENE= }} | | {{STRUCTURE_1qcm| PDB=1qcm | SCENE= }} |
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| - | '''AMYLOID BETA PEPTIDE (25-35), NMR, 20 STRUCTURES'''
| + | ===AMYLOID BETA PEPTIDE (25-35), NMR, 20 STRUCTURES=== |
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| - | ==Overview==
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| - | The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 experimental constraints, including 106 distance constraints reduced from the nuclear Overhauser effect (NOE) connectivities and four torsion angle (phi) constraints. The atomic root mean square difference about averaged coordinates is 1.04 +/- 0.25 A for the backbone atoms (N, C alpha, C) and 1.39 +/- 0.27 A for all heavy atoms of the entire peptide. The molecular structure of amyloid beta peptide in membrane-mimicking environment is composed of a short alpha helix in the C terminal position. The three residues from the N-terminus are disordered, but the remaining eight C-terminal residues are well-ordered, which is supported by the RMSD values of the C-terminal region, Lys28-Leu34. In this region, the RMS differences from averaged coordinates are 0.26 +/- 0.11 A for the backbone atoms (N, C alpha, C) and 0.77 +/- 0.21 A for all heavy atoms, which is very low compared with those for the entire peptide. The four amino acid residues from the N-terminus are hydrophilic and the other seven amino acid residues in C-terminus are hydrophobic. So, our results show that the C-terminal region of amyloid beta peptide (25-35) is buried in the membrane and assumes alpha-helical structure, whereas the N-terminal region is exposed to the solvent with a flexible structure. This structure is very similar to membrane-mediated structure of substance P previously reported. The three-dimensional structure of a non-neurotoxic mutant of amyloid beta peptide (25-35), where Asn27 is replaced by Ala, in lithium dodecyl sulfate micelles was also determined. The structure is similar to that of the wild type amyloid beta peptide (25-35) in the C-terminal region, but the N-terminal flexible region is different. The structural comparison of amyloid beta peptide (25-35), its non-neurotoxic mutant and substance P gives a structural basis to understand the mechanism of neurotoxicity caused by amyloid beta peptide. | + | The line below this paragraph, {{ABSTRACT_PUBMED_8973180}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8973180 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8973180}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1QCM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCM OCA]. | + | 1QCM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCM OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Signal]] | | [[Category: Signal]] |
| | [[Category: Transmembrane]] | | [[Category: Transmembrane]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:07:56 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:36:07 2008'' |
Revision as of 16:36, 27 July 2008
Template:STRUCTURE 1qcm
AMYLOID BETA PEPTIDE (25-35), NMR, 20 STRUCTURES
Template:ABSTRACT PUBMED 8973180
About this Structure
1QCM is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
Three-dimensional structures of the amyloid beta peptide (25-35) in membrane-mimicking environment., Kohno T, Kobayashi K, Maeda T, Sato K, Takashima A, Biochemistry. 1996 Dec 17;35(50):16094-104. PMID:8973180
Page seeded by OCA on Sun Jul 27 19:36:07 2008
