1qd0

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[[Image:1qd0.gif|left|200px]]
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{{STRUCTURE_1qd0| PDB=1qd0 | SCENE= }}
{{STRUCTURE_1qd0| PDB=1qd0 | SCENE= }}
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'''CAMELID HEAVY CHAIN VARIABLE DOMAINS PROVIDE EFFICIENT COMBINING SITES TO HAPTENS'''
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===CAMELID HEAVY CHAIN VARIABLE DOMAINS PROVIDE EFFICIENT COMBINING SITES TO HAPTENS===
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==Overview==
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Camelids can produce antibodies devoid of light chains and CH1 domains (Hamers-Casterman, C. et al. (1993) Nature 363, 446-448). Camelid heavy-chain variable domains (VHH) have high affinities for protein antigens and the structures of two of these complexes have been determined (Desmyter, A. et al. (1996) Nature Struc. Biol. 3, 803-811; Decanniere, K. et al. (1999) Structure 7, 361-370). However, the small size of these VHHs and their monomeric nature bring into question their capacity to bind haptens. Here, we have successfully raised llama antibodies against the hapten azo-dye Reactive Red (RR6) and determined the crystal structure of the complex between a dimer of this hapten and a VHH fragment. The surface of interaction between the VHH and the dimeric hapten is large, with an area of ca. 300 A(2); this correlates well with the low-dissociation constant of 22 nM measured for the monomer. The VHH fragment provides an efficient combining site to the RR6, using its three CDR loops. In particular, CDR1 provides a strong interaction to the hapten through two histidine residues bound to its copper atoms. VHH fragments might, therefore, prove to be valuable tools for selecting, removing, or capturing haptens. They are likely to play a role in biotechnology extending beyond protein recognition alone.
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(as it appears on PubMed at http://www.pubmed.gov), where 10684599 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10684599}}
==About this Structure==
==About this Structure==
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[[Category: Immune system]]
[[Category: Immune system]]
[[Category: Immunoglobulin fragment]]
[[Category: Immunoglobulin fragment]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:08:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:04:42 2008''

Revision as of 12:04, 28 July 2008

Image:1qd0.png

Template:STRUCTURE 1qd0

CAMELID HEAVY CHAIN VARIABLE DOMAINS PROVIDE EFFICIENT COMBINING SITES TO HAPTENS

Template:ABSTRACT PUBMED 10684599

About this Structure

1QD0 is a Single protein structure of sequence from Lama glama. Full crystallographic information is available from OCA.

Reference

Camelid heavy-chain variable domains provide efficient combining sites to haptens., Spinelli S, Frenken LG, Hermans P, Verrips T, Brown K, Tegoni M, Cambillau C, Biochemistry. 2000 Feb 15;39(6):1217-22. PMID:10684599

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