8xid

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Current revision (06:15, 30 October 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8xid is ON HOLD until Paper Publication
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==DGNNV protrusion domain structure at neutral pH==
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<StructureSection load='8xid' size='340' side='right'caption='[[8xid]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8xid]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dragon_grouper_nervous_necrosis_virus Dragon grouper nervous necrosis virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XID OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XID FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xid OCA], [https://pdbe.org/8xid PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xid RCSB], [https://www.ebi.ac.uk/pdbsum/8xid PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xid ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9E6H7_9VIRU Q9E6H7_9VIRU]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many viruses contain surface spikes or protrusions that are essential for virus entry. These surface structures can thereby be targeted by antiviral drugs to treat viral infections. Nervous necrosis virus (NNV), a simple nonenveloped virus in the genus of betanodavirus, infects fish and damages aquaculture worldwide. NNV has 60 conspicuous surface protrusions, each comprising three protrusion domains (P-domain) of its capsid protein. NNV uses protrusions to bind to common receptors of sialic acids on the host cell surface to initiate its entry via the endocytic pathway. However, structural alterations of NNV in response to acidic conditions encountered during this pathway remain unknown, while detailed interactions of protrusions with receptors are unclear. Here, we used cryo-EM to discover that Grouper NNV protrusions undergo low-pH-induced compaction and resting. NMR and molecular dynamics (MD) simulations were employed to probe the atomic details. A solution structure of the P-domain at pH 7.0 revealed a long flexible loop (amino acids 311-330) and a pocket outlined by this loop. Molecular docking analysis showed that the N-terminal moiety of sialic acid inserted into this pocket to interact with conserved residues inside. MD simulations demonstrated that part of this loop converted to a beta-strand under acidic conditions, allowing for P-domain trimerization and compaction. Additionally, a low-pH-favored conformation is attained for the linker connecting the P-domain to the NNV shell, conferring resting protrusions. Our findings uncover novel pH-dependent conformational switching mechanisms underlying NNV protrusion dynamics potentially utilized for facilitating NNV entry, providing new structural insights into complex NNV-host interactions with the identification of putative druggable hotspots on the protrusion.
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Authors:
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Molecular Mechanism of pH-Induced Protrusion Configuration Switching in Piscine Betanodavirus Implies a Novel Antiviral Strategy.,Sterbova P, Wang CH, Carillo KJD, Lou YC, Kato T, Namba K, Tzou DM, Chang WH ACS Infect Dis. 2024 Aug 1. doi: 10.1021/acsinfecdis.4c00407. PMID:39087906<ref>PMID:39087906</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 8xid" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Dragon grouper nervous necrosis virus]]
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[[Category: Large Structures]]
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[[Category: Chang WH]]
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[[Category: Sterbova P]]
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[[Category: Tzou DL]]

Current revision

DGNNV protrusion domain structure at neutral pH

PDB ID 8xid

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