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1qdt

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[[Image:1qdt.jpg|left|200px]]
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{{Seed}}
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{{STRUCTURE_1qdt| PDB=1qdt | SCENE= }}
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'''2.1 A RESOLUTION STRUCTURE OF ESCHERICHIA COLI LYTIC TRANSGLYCOYSLASE SLT35 IN COMPLEX WITH CALCIUM'''
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===2.1 A RESOLUTION STRUCTURE OF ESCHERICHIA COLI LYTIC TRANSGLYCOYSLASE SLT35 IN COMPLEX WITH CALCIUM===
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==Overview==
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The Escherichia coli lytic transglycosylase Slt35 contains a single metal ion-binding site that resembles EF-hand calcium-binding sites. The Slt35 EF-hand is only the second observation of such a domain in a prokaryotic protein. Two crystal structures at 2.1 A resolution show that both Ca2+ ions and Na+ ions can bind to the EF-hand domain, but in subtly different configurations. Heat-induced unfolding studies demonstrate that Ca2+ ions are preferentially bound, and that only Ca2+ ions significantly increase the melting temperature of Slt35. This shows that the EF-hand calcium-binding domain is important for the stability of Slt35.
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(as it appears on PubMed at http://www.pubmed.gov), where 10570954 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10570954}}
==About this Structure==
==About this Structure==
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[[Category: Ef-hand]]
[[Category: Ef-hand]]
[[Category: Glycosyl transferase]]
[[Category: Glycosyl transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:10:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:26:34 2008''

Revision as of 20:26, 27 July 2008

Image:1qdt.png

Template:STRUCTURE 1qdt

2.1 A RESOLUTION STRUCTURE OF ESCHERICHIA COLI LYTIC TRANSGLYCOYSLASE SLT35 IN COMPLEX WITH CALCIUM

Template:ABSTRACT PUBMED 10570954

About this Structure

1QDT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Binding of calcium in the EF-hand of Escherichia coli lytic transglycosylase Slt35 is important for stability., van Asselt EJ, Dijkstra BW, FEBS Lett. 1999 Sep 24;458(3):429-35. PMID:10570954

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