1shz
From Proteopedia
(New page: 200px<br /> <applet load="1shz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1shz, resolution 2.85Å" /> '''Crystal Structure o...) |
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| - | [[Image:1shz.gif|left|200px]]<br /> | + | [[Image:1shz.gif|left|200px]]<br /><applet load="1shz" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1shz, resolution 2.85Å" /> | caption="1shz, resolution 2.85Å" /> | ||
'''Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera'''<br /> | '''Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera'''<br /> | ||
==Overview== | ==Overview== | ||
| - | p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is | + | p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric Galpha subunits. The GAP function of p115RhoGEF resides within the N-terminal region of p115RhoGEF (the rgRGS domain), which includes a module that is structurally similar to RGS (regulators of G-protein signaling) domains. We present here the crystal structure of the rgRGS domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1. Two distinct surfaces of rgRGS interact with Galpha. The N-terminal betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate contacts with the catalytic site of Galpha. The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus/rattus_norvegicus Mus musculus/rattus norvegicus] with MG, ALF and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1SHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus/rattus_norvegicus Mus musculus/rattus norvegicus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ALF:'>ALF</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chen, Z.]] | [[Category: Chen, Z.]] | ||
| - | [[Category: Singer, W | + | [[Category: Singer, W D.]] |
| - | [[Category: Sprang, S | + | [[Category: Sprang, S R.]] |
| - | [[Category: Sternweis, P | + | [[Category: Sternweis, P C.]] |
[[Category: ALF]] | [[Category: ALF]] | ||
[[Category: GDP]] | [[Category: GDP]] | ||
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[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:41 2008'' |
Revision as of 13:01, 21 February 2008
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Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera
Contents |
Overview
p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric Galpha subunits. The GAP function of p115RhoGEF resides within the N-terminal region of p115RhoGEF (the rgRGS domain), which includes a module that is structurally similar to RGS (regulators of G-protein signaling) domains. We present here the crystal structure of the rgRGS domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1. Two distinct surfaces of rgRGS interact with Galpha. The N-terminal betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate contacts with the catalytic site of Galpha. The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13.
Disease
Known disease associated with this structure: Slowed nerve conduction velocity, AD OMIM:[608136]
About this Structure
1SHZ is a Protein complex structure of sequences from Homo sapiens and Mus musculus/rattus norvegicus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator., Chen Z, Singer WD, Sternweis PC, Sprang SR, Nat Struct Mol Biol. 2005 Feb;12(2):191-7. Epub 2005 Jan 16. PMID:15665872
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