1qhg

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{{STRUCTURE_1qhg| PDB=1qhg | SCENE= }}
{{STRUCTURE_1qhg| PDB=1qhg | SCENE= }}
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'''STRUCTURE OF DNA HELICASE MUTANT WITH ADPNP'''
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===STRUCTURE OF DNA HELICASE MUTANT WITH ADPNP===
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==Overview==
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Based upon the crystal structures of PcrA helicase, we have made and characterised mutations in a number of conserved helicase signature motifs around the ATPase active site. We have also determined structures of complexes of wild-type PcrA with ADPNP and of a mutant PcrA complexed with ADPNP and Mn2+. The kinetic and structural data define roles for a number of different residues in and around the ATP binding site. More importantly, our results also show that there are two functionally distinct conformations of ATP in the active site. In one conformation, ATP is hydrolysed poorly whereas in the other (activated) conformation, ATP is hydrolysed much more rapidly. We propose a mechanism to explain how the stimulation of ATPase activity afforded by binding of single-stranded DNA stabilises the activated conformation favouring Mg2+binding and a consequent repositioning of the gamma-phosphate group which promotes ATP hydrolysis. A part of the associated conformational change in the protein forces the side-chain of K37 to vacate the Mg2+binding site, allowing the cation to bind and interact with ATP.
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(as it appears on PubMed at http://www.pubmed.gov), where 10388562 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10388562}}
==About this Structure==
==About this Structure==
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[[Category: Helicase]]
[[Category: Helicase]]
[[Category: Sos response]]
[[Category: Sos response]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:16:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:47:27 2008''

Revision as of 07:47, 28 July 2008

Image:1qhg.png

Template:STRUCTURE 1qhg

STRUCTURE OF DNA HELICASE MUTANT WITH ADPNP

Template:ABSTRACT PUBMED 10388562

About this Structure

1QHG is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase., Soultanas P, Dillingham MS, Velankar SS, Wigley DB, J Mol Biol. 1999 Jul 2;290(1):137-48. PMID:10388562

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