1uol
From Proteopedia
(New page: 200px<br /> <applet load="1uol" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uol, resolution 1.9Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1UOL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UOL OCA]]. | + | 1UOL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: ZNA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UOL OCA]]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: tumor suppressor]] | [[Category: tumor suppressor]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:52:06 2007'' |
Revision as of 09:47, 30 October 2007
|
CRYSTAL STRUCTURE OF THE HUMAN P53 CORE DOMAIN MUTANT M133L/V203A/N239Y/N268D AT 1.9 A RESOLUTION.
Overview
Most of the cancer-associated mutations in the tumor suppressor p53 map to, its DNA-binding core domain. Many of them inactivate p53 by decreasing its, thermodynamic stability. We have previously designed the superstable, quadruple mutant M133L/V203A/N239Y/N268D containing the second-site, suppressor mutations N239Y and N268D, which specifically restore activity, and stability in several oncogenic mutants. Here we present the x-ray, structure of this quadruple mutant at 1.9 A resolution, which was solved, in a new crystal form in the absence of DNA. This structure reveals that, the four point mutations cause only small local structural changes, whereas the overall structure of the central beta-sandwich and the, DNA-binding surface is conserved. The suppressor mutation N268D results in, an ... [(full description)]
About this Structure
1UOL is a [Single protein] structure of sequence from [Homo sapiens] with ZN as [ligand]. Structure known Active Site: ZNA. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a superstable mutant of human p53 core domain. Insights into the mechanism of rescuing oncogenic mutations., Joerger AC, Allen MD, Fersht AR, J Biol Chem. 2004 Jan 9;279(2):1291-6. Epub 2003 Oct 8. PMID:14534297
Page seeded by OCA on Tue Oct 30 11:52:06 2007
