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| - | [[Image:1qk1.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1qk1| PDB=1qk1 | SCENE= }} | | {{STRUCTURE_1qk1| PDB=1qk1 | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF HUMAN UBIQUITOUS MITOCHONDRIAL CREATINE KINASE'''
| + | ===CRYSTAL STRUCTURE OF HUMAN UBIQUITOUS MITOCHONDRIAL CREATINE KINASE=== |
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| - | ==Overview==
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| - | Creatine kinase (CK), catalyzing the reversible trans-phosphorylation between ATP and creatine, plays a key role in the energy metabolism of cells with high and fluctuating energy requirements. We have solved the X-ray structure of octameric human ubiquitous mitochondrial CK (uMtCK) at 2.7 A resolution, representing the first human CK structure. The structure is very similar to the previously determined structure of sarcomeric mitochondrial CK (sMtCK). The cuboidal octamer has 422 point group symmetry with four dimers arranged along the fourfold axis and a central channel of approximately 20 A diameter, which extends through the whole octamer. Structural differences with respect to sMtCK are found in isoform-specific regions important for octamer formation and membrane binding. Octameric uMtCK is stabilized by numerous additional polar interactions between the N-termini of neighboring dimers, which extend into the central channel and form clamp-like structures, and by a pair of salt bridges in the hydrophobic interaction patch. The five C-terminal residues of uMtCK, carrying positive charges likely to be involved in phospholipid-binding, are poorly defined by electron density, indicating a more flexible region than the corresponding one in sMtCK. The structural differences between uMtCK and sMtCK are consistent with biochemical studies on octamer stability and membrane binding of the two isoforms.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10737943}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10737943 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10737943}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Mitochondrial permeability transition]] | | [[Category: Mitochondrial permeability transition]] |
| | [[Category: Octamer stability]] | | [[Category: Octamer stability]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:22:08 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:49:29 2008'' |
Revision as of 08:49, 29 July 2008
Template:STRUCTURE 1qk1
CRYSTAL STRUCTURE OF HUMAN UBIQUITOUS MITOCHONDRIAL CREATINE KINASE
Template:ABSTRACT PUBMED 10737943
About this Structure
1QK1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human ubiquitous mitochondrial creatine kinase., Eder M, Fritz-Wolf K, Kabsch W, Wallimann T, Schlattner U, Proteins. 2000 May 15;39(3):216-25. PMID:10737943
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