8jqc

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Current revision (09:46, 17 October 2024) (edit) (undo)
 
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==Novel Anti-phage System==
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==Structure of Gabija GajA-GajB 4:1 complex==
<StructureSection load='8jqc' size='340' side='right'caption='[[8jqc]], [[Resolution|resolution]] 3.39&Aring;' scene=''>
<StructureSection load='8jqc' size='340' side='right'caption='[[8jqc]], [[Resolution|resolution]] 3.39&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jqc OCA], [https://pdbe.org/8jqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jqc RCSB], [https://www.ebi.ac.uk/pdbsum/8jqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jqc ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jqc OCA], [https://pdbe.org/8jqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jqc RCSB], [https://www.ebi.ac.uk/pdbsum/8jqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jqc ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/GAJA_BACC6 GAJA_BACC6] Component of antiviral defense system Gabija type I, composed of GajA and GajB. Expression of Gabija type I in B.subtilis (strain BEST7003) confers resistance to phages phi105, phi29, rho14, SpBeta and SBSphiC (PubMed:29371424). An endonuclease that nicks double-stranded DNA within the sequence 5'-TNNNCGGGNNA-3' in the absence of nucleotides (NTP, dNTP and NDPs), cleaving after C-1. Has no detected ATPase activity. Expression of Gabija type I in E.coli B (strain ATCC 11303) confers resistance to phage T7 (PubMed:33885789). It is thought that this enzyme is strongly suppressed during physiological growth (in E.coli total nucleotide concentration is over 8.7 mM in mid-log phase), but during viral replication, when nucleotides are rapidly consumed, it is de-suppressed and degrades target DNA (Probable).<ref>PMID:29371424</ref> <ref>PMID:33885789</ref> <ref>PMID:33885789</ref>
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== Publication Abstract from PubMed ==
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Prokaryotes have evolved intricate innate immune systems against phage infection(1-7). Gabija is a highly widespread prokaryotic defence system that consists of two components, GajA and GajB(8). GajA functions as a DNA endonuclease that is inactive in the presence of ATP(9). Here, to explore how the Gabija system is activated for anti-phage defence, we report its cryo-electron microscopy structures in five states, including apo GajA, GajA in complex with DNA, GajA bound by ATP, apo GajA-GajB, and GajA-GajB in complex with ATP and Mg(2+). GajA is a rhombus-shaped tetramer with its ATPase domain clustered at the centre and the topoisomerase-primase (Toprim) domain located peripherally. ATP binding at the ATPase domain stabilizes the insertion region within the ATPase domain, keeping the Toprim domain in a closed state. Upon ATP depletion by phages, the Toprim domain opens to bind and cleave the DNA substrate. GajB, which docks on GajA, is activated by the cleaved DNA, ultimately leading to prokaryotic cell death. Our study presents a mechanistic landscape of Gabija activation.
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Structures and activation mechanism of the Gabija anti-phage system.,Li J, Cheng R, Wang Z, Yuan W, Xiao J, Zhao X, Du X, Xia S, Wang L, Zhu B, Wang L Nature. 2024 May;629(8011):467-473. doi: 10.1038/s41586-024-07270-x. Epub 2024 , Mar 12. PMID:38471529<ref>PMID:38471529</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 8jqc" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>

Current revision

Structure of Gabija GajA-GajB 4:1 complex

PDB ID 8jqc

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