This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qkg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1qkg.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1qkg.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1qkg| PDB=1qkg | SCENE= }}
{{STRUCTURE_1qkg| PDB=1qkg | SCENE= }}
-
'''DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT'''
+
===DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT===
-
==Overview==
+
<!--
-
In contrast to the highly mutagenic pyrimidine(6-4)pyrimidone photoproduct, its Dewar valence isomer (Dewar product) has low mutagenic potential and produces a broad range of mutations [LeClerc, J. E., Borden, A. &amp; Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the mutagenic property of the Dewar product, we used experimental NMR restraints and molecular dynamics to determine the solution structure of a Dewar-lesion DNA decamer duplex. This DNA decamer duplex (DW/GA duplex) contains a mismatched base pair between the 3' T residue of the Dewar lesion (T6) and an opposed G residue (G15). The 3' T (T6) of the Dewar lesion formed stable hydrogen bonds with the opposing G15 residue. However, the helical bending and unwinding angles of the DW/GA duplex were much larger than those of a second duplex that contains the Dewar lesion and opposing A15 and A16 residues (DW/AA duplex). The DW/GA duplex showed poorer stacking interactions at the two bases of the Dewar product and at the adjacent A7 small middle dotT14 base pair than did the DW/AA duplex. These structural features imply that no thermal stability or conformational benefit is obtained by incorporating a G instead of an A opposite the 3' T of the Dewar lesion. These properties may thus facilitate the preferential incorporation of an A in accordance with the A rule during translesion replication and lead to the low frequency of 3' T--&gt;C mutations observed at this site.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10758155}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10758155 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10758155}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QKG OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QKG OCA].
==Reference==
==Reference==
Line 29: Line 33:
[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Translesion replication]]
[[Category: Translesion replication]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:22:56 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:17:18 2008''

Revision as of 06:17, 29 July 2008

Image:1qkg.png

Template:STRUCTURE 1qkg

DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT

Template:ABSTRACT PUBMED 10758155

About this Structure

Full experimental information is available from OCA.

Reference

The Dewar photoproduct of thymidylyl(3'-->5')- thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"., Lee JH, Bae SH, Choi BS, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4591-6. PMID:10758155

Page seeded by OCA on Tue Jul 29 09:17:18 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qkg"
Personal tools