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1qp9

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{{STRUCTURE_1qp9| PDB=1qp9 | SCENE= }}
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'''STRUCTURE OF HAP1-PC7 COMPLEXED TO THE UAS OF CYC7'''
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===STRUCTURE OF HAP1-PC7 COMPLEXED TO THE UAS OF CYC7===
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==Overview==
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HAP1 is a transcription factor in yeast whose DNA-binding domain has been implicated in directly affecting transcriptional activation. Two separate mutations in the DNA-binding domain, S63G (HAP1-PC7) and S63R (HAP1-18), retain wild-type binding affinity. However, HAP1-PC7 is transcriptionally silent while HAP1-18 shows highly elevated levels of transcription. We have determined the X-ray crystal structure of the DNA-binding domain of HAP1-PC7 bound to its DNA target, UAS(CYC7), and compared it to the previously solved HAP1-wt and HAP1-18 complexes to UAS(CYC7). Additionally, we have quantitatively compared the DNA-binding affinity and specificity of the HAP1-PC7, HAP1-18 and HAP1-wt DNA-binding domains. We show that, although the DNA-binding domains of these three proteins bind UAS(CYC7) with comparable affinity and specificity, the protein-DNA interactions are dramatically different between the three complexes. Conserved protein-DNA interactions are largely restricted to an internal DNA sequence that excludes one of the two conserved DNA half-sites of UAS(CYC7) suggesting a mode of recognition distinct from other HAP1 family members. Alternative protein-DNA interactions result in divergent DNA configurations between the three complexes. These results suggest that the differential transcriptional activities of the HAP1, HAP1-18 and HAP1-PC7 proteins are due, at least in part, to alternative protein-DNA contacts, and implies that HAP1-DNA interactions have direct allosteric effects on transcriptional activation.
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{{ABSTRACT_PUBMED_11024163}}
==About this Structure==
==About this Structure==
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[[Category: Heptad repeat]]
[[Category: Heptad repeat]]
[[Category: Zinc binuclear cluster]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:49:53 2008''

Revision as of 02:49, 28 July 2008

Image:1qp9.png

Template:STRUCTURE 1qp9

STRUCTURE OF HAP1-PC7 COMPLEXED TO THE UAS OF CYC7

Template:ABSTRACT PUBMED 11024163

About this Structure

1QP9 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of HAP1-PC7 bound to DNA: implications for DNA recognition and allosteric effects of DNA-binding on transcriptional activation., Lukens AK, King DA, Marmorstein R, Nucleic Acids Res. 2000 Oct 15;28(20):3853-63. PMID:11024163

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