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| - | [[Image:1qt8.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1qt8| PDB=1qt8 | SCENE= }} | | {{STRUCTURE_1qt8| PDB=1qt8 | SCENE= }} |
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| - | '''T26H Mutant of T4 Lysozyme'''
| + | ===T26H Mutant of T4 Lysozyme=== |
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| - | ==Overview==
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| - | In contrast to hen egg-white lysozyme, which retains the beta-configuration of the substrate in the product, T4 lysozyme (T4L) is an inverting glycosidase. The substitution Thr-26 --> His, however, converts T4L from an inverting to a retaining enzyme. It is shown here that the Thr-26 --> His mutant is also a transglycosidase. Indeed, the transglycosylation reaction can be more effective than hydrolysis. In contrast, wild-type T4L has no detectable transglycosidase activity. The results support the prior hypothesis that catalysis by the Thr-26 --> His mutant proceeds via a covalent intermediate. Further mutations (Glu-11 --> His, Asp-20 --> Cys) of the T26H mutant lysozyme indicate that the catalytic mechanism of this mutant requires Glu-11 as a general acid but Asp-20 is not essential. The results help provide an overall rationalization for the activity of glycosidases, in which a highly conserved acid group (Glu-11 in T4L, Glu-35 in hen egg-white lysozyme) on the beta-side of the substrate acts as a proton donor, whereas alterations in the placement and chemical identity of residues on the alpha-side of the substrate can lead to catalysis with or without retention of the configuration, to transglycosidase activity, or to the formation of a stable enzyme-substrate adduct.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10430876}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10430876 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10430876}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Weaver, L H.]] | | [[Category: Weaver, L H.]] |
| | [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:40:46 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:34:28 2008'' |
Revision as of 04:34, 28 July 2008
Template:STRUCTURE 1qt8
T26H Mutant of T4 Lysozyme
Template:ABSTRACT PUBMED 10430876
About this Structure
1QT8 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Structural basis of the conversion of T4 lysozyme into a transglycosidase by reengineering the active site., Kuroki R, Weaver LH, Matthews BW, Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8949-54. PMID:10430876
Page seeded by OCA on Mon Jul 28 07:34:28 2008
