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1quq

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[[Image:1quq.gif|left|200px]]
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{{STRUCTURE_1quq| PDB=1quq | SCENE= }}
{{STRUCTURE_1quq| PDB=1quq | SCENE= }}
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'''COMPLEX OF REPLICATION PROTEIN A SUBUNITS RPA14 AND RPA32'''
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===COMPLEX OF REPLICATION PROTEIN A SUBUNITS RPA14 AND RPA32===
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==Overview==
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Replication protein A (RPA), the eukaryote single-stranded DNA-binding protein (SSB), is a heterotrimer. The largest subunit, RPA70, which harbours the major DNA-binding activity, has two DNA-binding domains that each adopt an OB-fold. The complex of the two smaller subunits, RPA32 and RPA14, has weak DNA-binding activity but the mechanism of DNA binding is unknown. We have determined the crystal structure of the proteolytic core of RPA32 and RPA14, which consists of the central two-thirds of RPA32 and the entire RPA14 subunit. The structure revealed that RPA14 and the central part of RPA32 are structural homologues. Each subunit contains a central OB-fold domain, which also resembles the DNA-binding domains in RPA70; an N-terminal extension that interacts with the central OB-fold domain; and a C-terminal helix that mediate heterodimerization via a helix-helix interaction. The OB-fold of RPA32, but not RPA14, possesses additional similarity to the RPA70 DNA-binding domains, supporting a DNA-binding role for RPA32. The discovery of a third and fourth OB-fold in RPA suggests that the quaternary structure of SSBs, which in Bacteria and Archaea are also tetramers of OB-folds, is conserved in evolution. The structure also suggests a mechanism for RPA trimer formation.
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{{ABSTRACT_PUBMED_10449415}}
==About this Structure==
==About this Structure==
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[[Category: Rpa]]
[[Category: Rpa]]
[[Category: Ssdna-binding]]
[[Category: Ssdna-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:43:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:52:42 2008''

Revision as of 10:52, 27 July 2008

Image:1quq.png

Template:STRUCTURE 1quq

COMPLEX OF REPLICATION PROTEIN A SUBUNITS RPA14 AND RPA32

Template:ABSTRACT PUBMED 10449415

About this Structure

1QUQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding., Bochkarev A, Bochkareva E, Frappier L, Edwards AM, EMBO J. 1999 Aug 16;18(16):4498-504. PMID:10449415

Page seeded by OCA on Sun Jul 27 13:52:42 2008

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