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1qvc

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[[Image:1qvc.gif|left|200px]]
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{{STRUCTURE_1qvc| PDB=1qvc | SCENE= }}
{{STRUCTURE_1qvc| PDB=1qvc | SCENE= }}
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'''CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI'''
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===CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI===
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==Overview==
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The single-stranded DNA (ssDNA) binding protein from Escherichia coli (EcoSSB) plays a central role in DNA replication, recombination and repair. The tertiary structure of EcoSSB was determined at 2.2 A resolution. This is rather higher resolution than previously reported. Crystals were grown from the homogeneous intact protein but the EcoSSB tetramer in the crystals contains truncated subunits lacking a part of the C-terminal. The structure determined includes biologically important flexible loops and C-terminal regions, and revealed the existence of concavities. These concavities include the residues important for ssDNA binding. An ssDNA can be fitted on the concavities and further stabilized through interactions with the loops forming flexible lids. It seems likely to play a central role in the binding of ssDNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_10731701}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10731701 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10731701}}
==About this Structure==
==About this Structure==
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[[Category: Beta-barrel]]
[[Category: Beta-barrel]]
[[Category: Single stranded dna binding protein]]
[[Category: Single stranded dna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:44:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:47:02 2008''

Revision as of 18:47, 27 July 2008

Image:1qvc.png

Template:STRUCTURE 1qvc

CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI

Template:ABSTRACT PUBMED 10731701

About this Structure

1QVC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis., Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N, J Biochem. 2000 Feb;127(2):329-35. PMID:10731701

Page seeded by OCA on Sun Jul 27 21:47:02 2008

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