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1qyo

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{{STRUCTURE_1qyo| PDB=1qyo | SCENE= }}
{{STRUCTURE_1qyo| PDB=1qyo | SCENE= }}
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'''Anaerobic precylization intermediate crystal structure for S65G Y66G GFP variant'''
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===Anaerobic precylization intermediate crystal structure for S65G Y66G GFP variant===
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==Overview==
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Green fluorescent protein has revolutionized cell labeling and molecular tagging, yet the driving force and mechanism for its spontaneous fluorophore synthesis are not established. Here we discover mutations that substantially slow the rate but not the yield of this posttranslational modification, determine structures of the trapped precyclization intermediate and oxidized postcyclization states, and identify unanticipated features critical to chromophore maturation. The protein architecture contains a dramatic approximately 80 degrees bend in the central helix, which focuses distortions at G67 to promote ring formation from amino acids S65, Y66, and G67. Significantly, these distortions eliminate potential helical hydrogen bonds that would otherwise have to be broken at an energetic cost during peptide cyclization and force the G67 nitrogen and S65 carbonyl oxygen atoms within van der Waals contact in preparation for covalent bond formation. Further, we determine that under aerobic, but not anaerobic, conditions the Gly-Gly-Gly chromophore sequence cyclizes and incorporates an oxygen atom. These results lead directly to a conjugation-trapping mechanism, in which a thermodynamically unfavorable cyclization reaction is coupled to an electronic conjugation trapping step, to drive chromophore maturation. Moreover, we propose primarily electrostatic roles for the R96 and E222 side chains in chromophore formation and suggest that the T62 carbonyl oxygen is the base that initiates the dehydration reaction. Our molecular mechanism provides the basis for understanding and eventually controlling chromophore creation.
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(as it appears on PubMed at http://www.pubmed.gov), where 14523232 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14523232}}
==About this Structure==
==About this Structure==
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[[Category: Chromophore]]
[[Category: Chromophore]]
[[Category: Trapped intermediate]]
[[Category: Trapped intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:51:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:17:15 2008''

Revision as of 13:17, 29 July 2008

Image:1qyo.png

Template:STRUCTURE 1qyo

Anaerobic precylization intermediate crystal structure for S65G Y66G GFP variant

Template:ABSTRACT PUBMED 14523232

About this Structure

1QYO is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures., Barondeau DP, Putnam CD, Kassmann CJ, Tainer JA, Getzoff ED, Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12111-6. Epub 2003 Oct 1. PMID:14523232

Page seeded by OCA on Tue Jul 29 16:17:15 2008

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