This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qzg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1qzg.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1qzg.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1qzg| PDB=1qzg | SCENE= }}
{{STRUCTURE_1qzg| PDB=1qzg | SCENE= }}
-
'''Crystal structure of Pot1 (protection of telomere)- ssDNA complex'''
+
===Crystal structure of Pot1 (protection of telomere)- ssDNA complex===
-
==Overview==
+
<!--
-
Telomeres, specialized protein-DNA complexes that cap the ends of linear chromosomes, are essential for protecting chromosomes from degradation and end-to-end fusions. The Pot1 (protection of telomeres 1) protein is a widely distributed eukaryotic end-capping protein, having been identified in fission yeast, microsporidia, plants and animals. Schizosaccharomyces pombe Pot1p is essential for telomere maintenance, and human POT1 has been implicated in telomerase regulation. Pot1 binds telomeric single-stranded DNA (ssDNA) with exceptionally high sequence specificity, the molecular basis of which has been unknown. Here we describe the 1.9-A-resolution crystal structure of the amino-terminal DNA-binding domain of S. pombe Pot1p complexed with ssDNA. The protein adopts an oligonucleotide/oligosaccharide-binding (OB) fold with two loops that protrude to form a clamp for ssDNA binding. The structure explains the sequence specificity of binding: in the context of the Pot1 protein, DNA self-recognition involving base-stacking and unusual G-T base pairs compacts the DNA. Any sequence change disrupts the ability of the DNA to form this structure, preventing it from contacting the array of protein hydrogen-bonding groups. The structure also explains how Pot1p avoids binding the vast excess of RNA in the nucleus.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14614509}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14614509 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14614509}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Protrein-dna complex]]
[[Category: Protrein-dna complex]]
[[Category: Single-stranded telomeric dna]]
[[Category: Single-stranded telomeric dna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:53:40 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:53:57 2008''

Revision as of 09:53, 28 July 2008

Image:1qzg.png

Template:STRUCTURE 1qzg

Crystal structure of Pot1 (protection of telomere)- ssDNA complex

Template:ABSTRACT PUBMED 14614509

About this Structure

1QZG is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.

Reference

DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA., Lei M, Podell ER, Baumann P, Cech TR, Nature. 2003 Nov 13;426(6963):198-203. PMID:14614509

Page seeded by OCA on Mon Jul 28 12:53:57 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qzg"
Personal tools