1ta6
From Proteopedia
(New page: 200px<br /> <applet load="1ta6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ta6, resolution 1.90Å" /> '''Crystal structure o...) |
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| - | [[Image:1ta6.gif|left|200px]]<br /> | + | [[Image:1ta6.gif|left|200px]]<br /><applet load="1ta6" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ta6" size=" | + | |
caption="1ta6, resolution 1.90Å" /> | caption="1ta6, resolution 1.90Å" /> | ||
'''Crystal structure of thrombin in complex with compound 14b'''<br /> | '''Crystal structure of thrombin in complex with compound 14b'''<br /> | ||
==Overview== | ==Overview== | ||
| - | As part of an ongoing effort to prepare therapeutically useful orally | + | As part of an ongoing effort to prepare therapeutically useful orally active thrombin inhibitors, we have synthesized a series of compounds that utilize nonbasic groups in the P1 position. The work is based on our previously reported lead structure, compound 1, which was discovered via a resin-based approach to varying P1. By minimizing the size and lipophilicity of the P3 group and by incorporating hydrogen-bonding groups on the N-terminus or on the 2-position of the P1 aromatic ring, we have prepared a number of derivatives in this series that exhibit subnanomolar enzyme potency combined with good in vivo antithrombotic and bioavailability profiles. The oxyacetic amide compound 14b exhibited the best overall profile of in vitro and in vivo activity, and crystallographic studies indicate a unique mode of binding in the thrombin active site. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with 177 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] Full crystallographic information is available from [http:// | + | 1TA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=177:'>177</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TA6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thrombin]] | [[Category: Thrombin]] | ||
| - | [[Category: Baskin, E | + | [[Category: Baskin, E P.]] |
| - | [[Category: Brady, S | + | [[Category: Brady, S F.]] |
| - | [[Category: Chen, I | + | [[Category: Chen, I W.]] |
| - | [[Category: Cook, J | + | [[Category: Cook, J J.]] |
| - | [[Category: Cooper, C | + | [[Category: Cooper, C M.]] |
| - | [[Category: Dancheck, K | + | [[Category: Dancheck, K B.]] |
| - | [[Category: Gardel, S | + | [[Category: Gardel, S J.]] |
| - | [[Category: Holahan, M | + | [[Category: Holahan, M A.]] |
| - | [[Category: Krueger, J | + | [[Category: Krueger, J A.]] |
| - | [[Category: Lewis, S | + | [[Category: Lewis, S D.]] |
| - | [[Category: Lucas, B | + | [[Category: Lucas, B Y.]] |
| - | [[Category: Lumma, W | + | [[Category: Lumma, W C.]] |
| - | [[Category: Lyle, E | + | [[Category: Lyle, E A.]] |
| - | [[Category: Lynch, J | + | [[Category: Lynch, J J.]] |
| - | [[Category: Naylor-Olsen, A | + | [[Category: Naylor-Olsen, A M.]] |
| - | [[Category: Sisko, J | + | [[Category: Sisko, J T.]] |
| - | [[Category: Stauffer, K | + | [[Category: Stauffer, K J.]] |
| - | [[Category: Stranieri, M | + | [[Category: Stranieri, M T.]] |
| - | [[Category: Tucker, T | + | [[Category: Tucker, T J.]] |
| - | [[Category: Vacca, J | + | [[Category: Vacca, J P.]] |
[[Category: Yan, Y.]] | [[Category: Yan, Y.]] | ||
[[Category: 177]] | [[Category: 177]] | ||
[[Category: thrombin inhibitor complex]] | [[Category: thrombin inhibitor complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:11:35 2008'' |
Revision as of 13:11, 21 February 2008
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Crystal structure of thrombin in complex with compound 14b
Contents |
Overview
As part of an ongoing effort to prepare therapeutically useful orally active thrombin inhibitors, we have synthesized a series of compounds that utilize nonbasic groups in the P1 position. The work is based on our previously reported lead structure, compound 1, which was discovered via a resin-based approach to varying P1. By minimizing the size and lipophilicity of the P3 group and by incorporating hydrogen-bonding groups on the N-terminus or on the 2-position of the P1 aromatic ring, we have prepared a number of derivatives in this series that exhibit subnanomolar enzyme potency combined with good in vivo antithrombotic and bioavailability profiles. The oxyacetic amide compound 14b exhibited the best overall profile of in vitro and in vivo activity, and crystallographic studies indicate a unique mode of binding in the thrombin active site.
Disease
Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]
About this Structure
1TA6 is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Thrombin, with EC number 3.4.21.5 Full crystallographic information is available from OCA.
Reference
Design and synthesis of a series of potent and orally bioavailable noncovalent thrombin inhibitors that utilize nonbasic groups in the P1 position., Tucker TJ, Brady SF, Lumma WC, Lewis SD, Gardell SJ, Naylor-Olsen AM, Yan Y, Sisko JT, Stauffer KJ, Lucas BJ, Lynch JJ, Cook JJ, Stranieri MT, Holahan MA, Lyle EA, Baskin EP, Chen IW, Dancheck KB, Krueger JA, Cooper CM, Vacca JP, J Med Chem. 1998 Aug 13;41(17):3210-9. PMID:9703466
Page seeded by OCA on Thu Feb 21 15:11:35 2008
Categories: Homo sapiens | Single protein | Thrombin | Baskin, E P. | Brady, S F. | Chen, I W. | Cook, J J. | Cooper, C M. | Dancheck, K B. | Gardel, S J. | Holahan, M A. | Krueger, J A. | Lewis, S D. | Lucas, B Y. | Lumma, W C. | Lyle, E A. | Lynch, J J. | Naylor-Olsen, A M. | Sisko, J T. | Stauffer, K J. | Stranieri, M T. | Tucker, T J. | Vacca, J P. | Yan, Y. | 177 | Thrombin inhibitor complex
