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1r1c

From Proteopedia

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{{STRUCTURE_1r1c| PDB=1r1c | SCENE= }}
{{STRUCTURE_1r1c| PDB=1r1c | SCENE= }}
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'''PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)'''
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===PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)===
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==Overview==
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Near-UV irradiation of structurally characterized [Re(I)(CO)3(1,10-phenanthroline)(Q107H)](W48F/Y72F/H83Q/Y108W)AzM(II) [Az = Pseudomonas aeruginosa azurin, M = Cu, Zn]/[Co(NH3)5Cl]Cl2 produces a tryptophan radical (W108*) with unprecedented kinetic stability. After rapid formation (k = 2.8 x 106 s-1), the radical persists for more than 5 h at room temperature in the folded ReAzM(II) structure. The absorption spectrum of ReAz(W108*)M(II) exhibits maxima at 512 and 536 nm. Oxidation of K4[Mo(CN)8] by ReAz(W108*)Zn(II) places the W108*/W108 reduction potential in the protein above 0.8 V vs NHE.
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{{ABSTRACT_PUBMED_14624538}}
==About this Structure==
==About this Structure==
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[[Category: Rhenium]]
[[Category: Rhenium]]
[[Category: Tunneling]]
[[Category: Tunneling]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:57:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:40:55 2008''

Revision as of 11:40, 29 July 2008

Image:1r1c.png

Template:STRUCTURE 1r1c

PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)

Template:ABSTRACT PUBMED 14624538

About this Structure

1R1C is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment., Miller JE, Gradinaru C, Crane BR, Di Bilio AJ, Wehbi WA, Un S, Winkler JR, Gray HB, J Am Chem Soc. 2003 Nov 26;125(47):14220-1. PMID:14624538

Page seeded by OCA on Tue Jul 29 14:40:55 2008

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