1r2n

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{{STRUCTURE_1r2n| PDB=1r2n | SCENE= }}
{{STRUCTURE_1r2n| PDB=1r2n | SCENE= }}
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'''NMR structure of the all-trans retinal in dark-adapted Bacteriorhodopsin'''
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===NMR structure of the all-trans retinal in dark-adapted Bacteriorhodopsin===
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==Overview==
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The two forms of bacteriorhodopsin present in the dark-adapted state, containing either all-trans or 13-cis,15-syn retinal, were examined by using solution state NMR, and their structures were determined. Comparison of the all-trans and the 13-cis,15-syn forms shows a shift in position of about 0.25 A within the pocket of the protein. Comparing this to the 13-cis,15-anti chromophore of the catalytic cycle M-intermediate structure, the 13-cis,15-syn form demonstrates a less pronounced up-tilt of the retinal C12[bond]C14 region, while leaving W182 and T178 essentially unchanged. The N[bond]H dipole of the Schiff base orients toward the extracellular side in both forms, however, it reorients toward the intracellular side in the 13-cis,15-anti configuration to form the catalytic M-intermediate. Thus, the change of the N[bond]H dipole is considered primarily responsible for energy storage, conformation changes of the protein, and the deprotonation of the Schiff base. The structural similarity of the all-trans and 13-cis,15-syn forms is taken as strong evidence for the ion dipole dragging model by which proton (hydroxide ion) translocation follows the change of the dipole.
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(as it appears on PubMed at http://www.pubmed.gov), where 12119389 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12119389}}
==About this Structure==
==About this Structure==
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1R2N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2N OCA].
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1R2N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2N OCA].
==Reference==
==Reference==
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[[Category: Proton transport]]
[[Category: Proton transport]]
[[Category: Retinal protein]]
[[Category: Retinal protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:00:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:59:24 2008''

Revision as of 06:59, 29 July 2008

Image:1r2n.png

Template:STRUCTURE 1r2n

NMR structure of the all-trans retinal in dark-adapted Bacteriorhodopsin

Template:ABSTRACT PUBMED 12119389

About this Structure

1R2N is a Single protein structure of sequence from Halobacterium salinarum. Full experimental information is available from OCA.

Reference

The structures of the active center in dark-adapted bacteriorhodopsin by solution-state NMR spectroscopy., Patzelt H, Simon B, terLaak A, Kessler B, Kuhne R, Schmieder P, Oesterhelt D, Oschkinat H, Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9765-70. Epub 2002 Jul 15. PMID:12119389

Page seeded by OCA on Tue Jul 29 09:59:24 2008

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