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1r4g

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{{STRUCTURE_1r4g| PDB=1r4g | SCENE= }}
{{STRUCTURE_1r4g| PDB=1r4g | SCENE= }}
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'''Solution structure of the Sendai virus protein X C-subdomain'''
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===Solution structure of the Sendai virus protein X C-subdomain===
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==Overview==
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The RNA-dependent RNA polymerase of the Sendai virus (SeV) consists of the large protein (L) and the phosphoprotein (P). P plays a crucial role in the enzyme by positioning L (which carries the polymerase activity) onto the matrix for transcription and replication formed by the RNA and the nucleoprotein, the N-RNA. P has a modular structure with distinct functional domains: an N-terminal domain involved in binding to N degrees (N that is not yet bound to RNA) and a C-terminal domain that carries the oligomerisation domain, the N-RNA binding domain and the L binding domain and that, combined with L, is active in transcription. Structural data have previously been obtained on the N-terminal domain and on the oligomerisation domain of P, but not yet on its N-RNA binding domain (also-called the X protein). Here we present an NMR and a small angle neutron scattering study of the SeV X protein. We show that this molecule presents two subdomains linked by an 11-residue linker, with the N-subdomain lacking a well-defined conformation. The 3D structure of the C-subdomain consists of three alpha-helices revealing an asymmetric charge distribution that may be important for binding to RNA-bound nucleoprotein. The structure of the entire C-terminal domain of P is modelled from its constituent parts in combination with small angle scattering data on this domain.
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(as it appears on PubMed at http://www.pubmed.gov), where 14980481 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14980481}}
==About this Structure==
==About this Structure==
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1R4G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sendai_virus Sendai virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4G OCA].
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1R4G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sendai_virus Sendai virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4G OCA].
==Reference==
==Reference==
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[[Category: Timmins, P.]]
[[Category: Timmins, P.]]
[[Category: Three helix-bundle]]
[[Category: Three helix-bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:03:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:50:50 2008''

Revision as of 05:50, 29 July 2008

Image:1r4g.png

Template:STRUCTURE 1r4g

Solution structure of the Sendai virus protein X C-subdomain

Template:ABSTRACT PUBMED 14980481

About this Structure

1R4G is a Single protein structure of sequence from Sendai virus. Full experimental information is available from OCA.

Reference

Structure and dynamics of the nucleocapsid-binding domain of the Sendai virus phosphoprotein in solution., Blanchard L, Tarbouriech N, Blackledge M, Timmins P, Burmeister WP, Ruigrok RW, Marion D, Virology. 2004 Feb 20;319(2):201-11. PMID:14980481

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