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1r6h

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[[Image:1r6h.gif|left|200px]]
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{{STRUCTURE_1r6h| PDB=1r6h | SCENE= }}
{{STRUCTURE_1r6h| PDB=1r6h | SCENE= }}
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'''Solution Structure of human PRL-3'''
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===Solution Structure of human PRL-3===
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==Overview==
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Phosphatases and kinases are the cellular signal transduction enzymes that control protein phosphorylation. PRL phosphatases constitute a novel class of small (20 kDa), prenylated phosphatases with oncogenic activity. In particular, PRL-3 is consistently overexpressed in liver metastasis in colorectal cancer cells and represents a new therapeutic target. Here, we present the solution structure of PRL-3, the first structure of a PRL phosphatase. The structure places PRL phosphatases in the class of dual specificity phosphatases with closest structural homology to the VHR phosphatase. The structure, coupled with kinetic studies of site-directed mutants, identifies functionally important residues and reveals unique features, differentiating PRLs from other phosphatases. These differences include an unusually hydrophobic active site without the catalytically important serine/threonine found in most other phosphatases. The position of the general acid loop indicates the presence of conformational change upon catalysis. The studies also identify a potential regulatory role of Cys(49) that forms an intramolecular disulfide bond with the catalytic Cys(104) even under mildly reducing conditions. Molecular modeling of the highly homologous PRL-1 and PRL-2 phosphatases revealed unique surface elements that are potentially important for specificity.
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(as it appears on PubMed at http://www.pubmed.gov), where 14704153 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14704153}}
==About this Structure==
==About this Structure==
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1R6H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R6H OCA].
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1R6H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R6H OCA].
==Reference==
==Reference==
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[[Category: Kozlov, G.]]
[[Category: Kozlov, G.]]
[[Category: Dual specificity phosphatase fold]]
[[Category: Dual specificity phosphatase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:08:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:49:11 2008''

Revision as of 00:49, 28 July 2008

Image:1r6h.png

Template:STRUCTURE 1r6h

Solution Structure of human PRL-3

Template:ABSTRACT PUBMED 14704153

About this Structure

1R6H is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structural insights into molecular function of the metastasis-associated phosphatase PRL-3., Kozlov G, Cheng J, Ziomek E, Banville D, Gehring K, Ekiel I, J Biol Chem. 2004 Mar 19;279(12):11882-9. Epub 2004 Jan 1. PMID:14704153

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