1r73

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[[Image:1r73.gif|left|200px]]
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{{STRUCTURE_1r73| PDB=1r73 | SCENE= }}
{{STRUCTURE_1r73| PDB=1r73 | SCENE= }}
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'''Solution Structure of TM1492, the L29 ribosomal protein from Thermotoga maritima'''
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===Solution Structure of TM1492, the L29 ribosomal protein from Thermotoga maritima===
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==Overview==
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This paper describes the NMR screening of 141 small (&lt;15 kDa) recombinant Thermotoga maritima proteins for globular folding. The experimental data shows that approximately 25% of the screened proteins are folded under our screening conditions, which makes this procedure an important step for selecting those proteins that are suitable for structure determination. A comparison of screening based either on 1D 1H NMR with unlabeled proteins or on 2D [1H,15N]-COSY with uniformly 15N-labeled proteins is presented, and a comprehensive analysis of the 1D 1H NMR screening data is described. As an illustration of the utility of these methods to structural proteomics, the NMR structure determination of TM1492 (ribosomal protein L29) is presented. This 66-residue protein consists of a N-terminal 3(10)-helix and two long alpha-helices connected by a tight turn centered about glycine 35, where conserved leucine and isoleucine residues in the two alpha-helices form a small hydrophobic core.
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{{ABSTRACT_PUBMED_15263836}}
==About this Structure==
==About this Structure==
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1R73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R73 OCA].
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1R73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R73 OCA].
==Reference==
==Reference==
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[[Category: Ribosome]]
[[Category: Ribosome]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:09:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:40:17 2008''

Revision as of 00:40, 29 July 2008

Image:1r73.png

Template:STRUCTURE 1r73

Solution Structure of TM1492, the L29 ribosomal protein from Thermotoga maritima

Template:ABSTRACT PUBMED 15263836

About this Structure

1R73 is a Single protein structure of sequence from Thermotoga maritima. Full experimental information is available from OCA.

Reference

NMR for structural proteomics of Thermotoga maritima: screening and structure determination., Peti W, Etezady-Esfarjani T, Herrmann T, Klock HE, Lesley SA, Wuthrich K, J Struct Funct Genomics. 2004;5(3):205-15. PMID:15263836

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