1r7f

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{{STRUCTURE_1r7f| PDB=1r7f | SCENE= }}
{{STRUCTURE_1r7f| PDB=1r7f | SCENE= }}
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'''NMR structure of the membrane anchor domain (1-31) of the nonstructural protein 5A (NS5A) of hepatitis C virus (Ensemble of 43 structures. Sample in 100mM SDS)'''
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===NMR structure of the membrane anchor domain (1-31) of the nonstructural protein 5A (NS5A) of hepatitis C virus (Ensemble of 43 structures. Sample in 100mM SDS)===
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==Overview==
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Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) is a membrane-associated, essential component of the viral replication complex. Here, we report the three-dimensional structure of the membrane anchor domain of NS5A as determined by NMR spectroscopy. An alpha-helix extending from amino acid residue 5 to 25 was observed in the presence of different membrane mimetic media. This helix exhibited a hydrophobic, Trprich side embedded in detergent micelles, while the polar, charged side was exposed to the solvent. Thus, the NS5A membrane anchor domain forms an in-plane amphipathic alpha-helix embedded in the cytosolic leaflet of the membrane bilayer. Interestingly, mutations affecting the positioning of fully conserved residues located at the cytosolic surface of the helix impaired HCV RNA replication without interfering with the membrane association of NS5A. In conclusion, the NS5A membrane anchor domain constitutes a unique platform that is likely involved in specific interactions essential for the assembly of the HCV replication complex and that may represent a novel target for antiviral intervention.
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(as it appears on PubMed at http://www.pubmed.gov), where 15247283 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15247283}}
==About this Structure==
==About this Structure==
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1R7F is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R7F OCA].
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1R7F is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R7F OCA].
==Reference==
==Reference==
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[[Category: Nmr structure]]
[[Category: Nmr structure]]
[[Category: Peptide.]]
[[Category: Peptide.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:10:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:09:12 2008''

Revision as of 11:09, 29 July 2008

Image:1r7f.png

Template:STRUCTURE 1r7f

NMR structure of the membrane anchor domain (1-31) of the nonstructural protein 5A (NS5A) of hepatitis C virus (Ensemble of 43 structures. Sample in 100mM SDS)

Template:ABSTRACT PUBMED 15247283

About this Structure

1R7F is a Single protein structure. Full experimental information is available from OCA.

Reference

Structure and function of the membrane anchor domain of hepatitis C virus nonstructural protein 5A., Penin F, Brass V, Appel N, Ramboarina S, Montserret R, Ficheux D, Blum HE, Bartenschlager R, Moradpour D, J Biol Chem. 2004 Sep 24;279(39):40835-43. Epub 2004 Jul 7. PMID:15247283

Page seeded by OCA on Tue Jul 29 14:09:12 2008

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