1rb7

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[[Image:1rb7.gif|left|200px]]
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{{Seed}}
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[[Image:1rb7.png|left|200px]]
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{{STRUCTURE_1rb7| PDB=1rb7 | SCENE= }}
{{STRUCTURE_1rb7| PDB=1rb7 | SCENE= }}
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'''Yeast cytosine deaminase crystal form p212121 with sodium acetate.'''
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===Yeast cytosine deaminase crystal form p212121 with sodium acetate.===
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==Overview==
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A crystallization strategy termed 'microseed matrix screening' is described where the optimal conditions for nucleation versus extended lattice growth are not compatible. This method is an extension of conventional seeding techniques in which microseeds from the nucleation step are systematically seeded into new conditions where all components of the drop are allowed to vary to screen for subsequent growth of well ordered specimens. The structure of a crystal form of yeast cytosine deaminase produced by streak-seeding using a single condition for both nucleation and growth is compared with the structure of a related crystal form produced by separating nucleation and growth conditions. The resulting structural comparison demonstrates that differential chelation patterns of cations by acidic surface residues of proteins within crystal lattice contacts is a critical parameter of crystal nucleation and growth.
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The line below this paragraph, {{ABSTRACT_PUBMED_14993707}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14993707 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14993707}}
==About this Structure==
==About this Structure==
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[[Category: Streak seeding]]
[[Category: Streak seeding]]
[[Category: Zinc bound]]
[[Category: Zinc bound]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:17:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:53:17 2008''

Revision as of 09:53, 29 July 2008

Image:1rb7.png

Template:STRUCTURE 1rb7

Yeast cytosine deaminase crystal form p212121 with sodium acetate.

Template:ABSTRACT PUBMED 14993707

About this Structure

1RB7 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Microseed matrix screening to improve crystals of yeast cytosine deaminase., Ireton GC, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):601-5. Epub 2004, Feb 25. PMID:14993707

Page seeded by OCA on Tue Jul 29 12:53:17 2008

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