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1rbo

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[[Image:1rbo.gif|left|200px]]
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{{STRUCTURE_1rbo| PDB=1rbo | SCENE= }}
{{STRUCTURE_1rbo| PDB=1rbo | SCENE= }}
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'''SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'''
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===SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE===
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==Overview==
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Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the carboxylation of ribulose 1,5-bisphosphate. The reaction catalyzed by Rubisco involves several steps, some of which can occur as partial reactions, forming intermediates that can be isolated. Analogues of these intermediates are potent inhibitors of the enzyme. We have studied the interactions with the enzyme of two inhibitors, xylulose 1,5-bisphosphate and 4-carboxyarabinitol 1,5-bisphosphate, by x-ray crystallography. Crystals of the complexes were formed by cocrystallization under activating conditions. In addition, 4-carboxyarabinitol 1,5-bisphosphate was soaked into preformed activated crystals of the enzyme. The result of these experiments was the release of the activating CO2 molecule as well as the metal ion from the active site when the inhibitors bound to the enzyme. Comparison with the structure of an activated complex of the enzyme indicates that the structural basis for the release of the activator groups is a distortion of the metal binding site due to the different geometry of the C-3 hydroxyl of the inhibitors. Both inhibitors induce closure of active site loops despite the inactivated state of the enzyme. Xylulose 1,5-bisphosphate binds in a hydrated form at the active site.
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(as it appears on PubMed at http://www.pubmed.gov), where 8955130 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8955130}}
==About this Structure==
==About this Structure==
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[[Category: Carbon-carbon]]
[[Category: Carbon-carbon]]
[[Category: Lyase]]
[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:18:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:18:21 2008''

Revision as of 06:18, 28 July 2008

Image:1rbo.png

Template:STRUCTURE 1rbo

SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE

Template:ABSTRACT PUBMED 8955130

About this Structure

1RBO is a Protein complex structure of sequences from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

A common structural basis for the inhibition of ribulose 1,5-bisphosphate carboxylase by 4-carboxyarabinitol 1,5-bisphosphate and xylulose 1,5-bisphosphate., Taylor TC, Fothergill MD, Andersson I, J Biol Chem. 1996 Dec 20;271(51):32894-9. PMID:8955130

Page seeded by OCA on Mon Jul 28 09:18:21 2008

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