1ttx
From Proteopedia
(New page: 200px<br /> <applet load="1ttx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ttx" /> '''Solution Stucture of human beta parvalbumin...) |
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| - | [[Image:1ttx.gif|left|200px]]<br /> | + | [[Image:1ttx.gif|left|200px]]<br /><applet load="1ttx" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ttx" size=" | + | |
caption="1ttx" /> | caption="1ttx" /> | ||
'''Solution Stucture of human beta parvalbumin (oncomodulin) refined with a paramagnetism based strategy'''<br /> | '''Solution Stucture of human beta parvalbumin (oncomodulin) refined with a paramagnetism based strategy'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The aim of this research was to determine the structure of human | + | The aim of this research was to determine the structure of human beta-parvalbumin (109 amino acids) and to compare it with its paralog and ortholog proteins. The structure was determined in solution using multinuclear and multidimensional NMR methods and refined using substitution of the EF-hand Ca(2+) ion with a paramagnetic lanthanide. The resulting family of structures had a backbone rmsd of 0.50 A. Comparison with rat oncomodulin (X-ray, 1.3 A resolution) as well as with human (NMR, backbone rmsd of 0.49 A) and rat (X-ray, 2.0 A resolution) parvalbumins reveals small but reliable local differences, often but not always related to amino acid variability. The analysis of these structures has led us to propose an explanation for the different affinity for Ca(2+) between alpha- and beta-parvalbumins and between parvalbumins and calmodulins. |
==About this Structure== | ==About this Structure== | ||
| - | 1TTX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1TTX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Babini, E.]] | [[Category: Babini, E.]] | ||
[[Category: Bertini, I.]] | [[Category: Bertini, I.]] | ||
| - | [[Category: Bianco, C | + | [[Category: Bianco, C Del.]] |
[[Category: Capozzi, F.]] | [[Category: Capozzi, F.]] | ||
[[Category: Hollender, D.]] | [[Category: Hollender, D.]] | ||
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[[Category: Luchinat, C.]] | [[Category: Luchinat, C.]] | ||
[[Category: Quattrone, A.]] | [[Category: Quattrone, A.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: calcium]] | [[Category: calcium]] | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:17:16 2008'' |
Revision as of 13:17, 21 February 2008
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Solution Stucture of human beta parvalbumin (oncomodulin) refined with a paramagnetism based strategy
Overview
The aim of this research was to determine the structure of human beta-parvalbumin (109 amino acids) and to compare it with its paralog and ortholog proteins. The structure was determined in solution using multinuclear and multidimensional NMR methods and refined using substitution of the EF-hand Ca(2+) ion with a paramagnetic lanthanide. The resulting family of structures had a backbone rmsd of 0.50 A. Comparison with rat oncomodulin (X-ray, 1.3 A resolution) as well as with human (NMR, backbone rmsd of 0.49 A) and rat (X-ray, 2.0 A resolution) parvalbumins reveals small but reliable local differences, often but not always related to amino acid variability. The analysis of these structures has led us to propose an explanation for the different affinity for Ca(2+) between alpha- and beta-parvalbumins and between parvalbumins and calmodulins.
About this Structure
1TTX is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of human beta-parvalbumin and structural comparison with its paralog alpha-parvalbumin and with their rat orthologs., Babini E, Bertini I, Capozzi F, Del Bianco C, Hollender D, Kiss T, Luchinat C, Quattrone A, Biochemistry. 2004 Dec 28;43(51):16076-85. PMID:15610002
Page seeded by OCA on Thu Feb 21 15:17:16 2008
Categories: Homo sapiens | Single protein | Babini, E. | Bertini, I. | Bianco, C Del. | Capozzi, F. | Hollender, D. | Kiss, T. | Luchinat, C. | Quattrone, A. | SPINE, Structural Proteomics in Europe. | CA | Calcium | Ef-hand | Lanthanide | Nmr | Oncomodulin | Spine | Structural genomics | Structural proteomics in europe
