This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1re0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1re0.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1re0.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1re0| PDB=1re0 | SCENE= }}
{{STRUCTURE_1re0| PDB=1re0 | SCENE= }}
-
'''Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A'''
+
===Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A===
-
==Overview==
+
<!--
-
ARF GTPases are activated by guanine nucleotide exchange factors (GEFs) of the Sec7 family that promote the exchange of GDP for GTP. Brefeldin A (BFA) is a fungal metabolite that binds to the ARF1*GDP*Sec7 complex and blocks GEF activity at an early stage of the reaction, prior to guanine nucleotide release. The crystal structure of the ARF1*GDP*Sec7*BFA complex shows that BFA binds at the protein-protein interface to inhibit conformational changes in ARF1 required for Sec7 to dislodge the GDP molecule. Based on a comparative analysis of the inhibited complex, nucleotide-free ARF1*Sec7 and ARF1*GDP, we suggest that, in addition to forcing nucleotide release, the ARF1-Sec7 binding energy is used to open a cavity on ARF1 to facilitate the rearrangement of hydrophobic core residues between the GDP and GTP conformations. Thus, the Sec7 domain may act as a dual catalyst, facilitating both nucleotide release and conformational switching on ARF proteins.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14690595}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14690595 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14690595}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: All-helical]]
[[Category: All-helical]]
[[Category: Alph-beta]]
[[Category: Alph-beta]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:22:56 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:35:41 2008''

Revision as of 09:35, 28 July 2008

Image:1re0.png

Template:STRUCTURE 1re0

Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A

Template:ABSTRACT PUBMED 14690595

About this Structure

1RE0 is a Protein complex structure of sequences from Homo sapiens and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism., Mossessova E, Corpina RA, Goldberg J, Mol Cell. 2003 Dec;12(6):1403-11. PMID:14690595

Page seeded by OCA on Mon Jul 28 12:35:41 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1re0"
Personal tools