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1rhc

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{{STRUCTURE_1rhc| PDB=1rhc | SCENE= }}
{{STRUCTURE_1rhc| PDB=1rhc | SCENE= }}
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'''F420-dependent secondary alcohol dehydrogenase in complex with an F420-acetone adduct'''
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===F420-dependent secondary alcohol dehydrogenase in complex with an F420-acetone adduct===
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==Overview==
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F(420)-dependent secondary alcohol dehydrogenase (Adf) from methanogenic archaea is a member of the growing bacterial luciferase family which are all TIM barrel enzymes, most of which with an unusual nonprolyl cis peptide bond. We report here on the crystal structure of Adf from Methanoculleus thermophilicus at 1.8 A resolution in complex with a F(420)-acetone adduct. The knowledge of the F(420) binding mode in Adf provides the molecular basis for modeling F(420) and FMN into the other enzymes of the family. A nonprolyl cis peptide bond was identified as an essential part of a bulge that serves as backstop at the Re-face of F(420) to keep it in a bent conformation. The acetone moiety of the F(420)-acetone adduct is positioned at the Si-face of F(420) deeply buried inside the protein. Isopropanol can be reliably modeled and a hydrogen transfer mechanism postulated. His39 and Glu108 can be identified as key players for binding of the acetone or isopropanol oxygens and for catalysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 15016352 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15016352}}
==About this Structure==
==About this Structure==
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[[Category: Thauer, R K.]]
[[Category: Thauer, R K.]]
[[Category: Warkentin, E.]]
[[Category: Warkentin, E.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:30:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:44:08 2008''

Revision as of 00:44, 28 July 2008

Image:1rhc.png

Template:STRUCTURE 1rhc

F420-dependent secondary alcohol dehydrogenase in complex with an F420-acetone adduct

Template:ABSTRACT PUBMED 15016352

About this Structure

1RHC is a Single protein structure of sequence from Methanoculleus thermophilus. Full crystallographic information is available from OCA.

Reference

Coenzyme binding in F420-dependent secondary alcohol dehydrogenase, a member of the bacterial luciferase family., Aufhammer SW, Warkentin E, Berk H, Shima S, Thauer RK, Ermler U, Structure. 2004 Mar;12(3):361-70. PMID:15016352

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