1rjx

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{{STRUCTURE_1rjx| PDB=1rjx | SCENE= }}
{{STRUCTURE_1rjx| PDB=1rjx | SCENE= }}
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'''Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT'''
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===Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT===
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==Overview==
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Streptokinase (SK) is a human plasminogen (Pg) activator secreted by streptococci. The activation mechanism of SK differs from that of physiological Pg activators in that SK is not a protease and cannot proteolytically activate Pg. Instead, it forms a tight complex with Pg that proteolytically activates other Pg molecules. The residue Lys-698 of human Pg was hypothesized to participate in triggering activation in the SK-Pg complex. Here, we report a study of the Lys-698 to Met substitution in the catalytic domain of Pg (microPg) containing the proteolytic activation-resistant background (R561A). While it remains competent in forming a complex with SK, maintaining a comparable equilibration dissociation constant (K(D)), the recombinant protein shows a nearly 60-fold reduction in amidolytic activity relative to its R561A background when mixed with native SK. A 2.3 A crystal structure of this mutant microPg confirmed the correct folding of this recombinant protein. Combined with other biochemical data, these results support the premise that Lys-698 of human Pg plays a functional role in the so-called N-terminal insertion activation mechanism by SK.
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(as it appears on PubMed at http://www.pubmed.gov), where 15211511 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15211511}}
==Disease==
==Disease==
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[[Category: Plasminogen activation]]
[[Category: Plasminogen activation]]
[[Category: Streptokinase]]
[[Category: Streptokinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:35:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:10:37 2008''

Revision as of 03:10, 29 July 2008

Image:1rjx.png

Template:STRUCTURE 1rjx

Contents

Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT

Template:ABSTRACT PUBMED 15211511

Disease

Known disease associated with this structure: Conjunctivitis, ligneous OMIM:[173350], Plasminogen Tochigi disease OMIM:[173350], Plasminogen deficiency, types I and II OMIM:[173350], Thrombophilia, dysplasminogenemic OMIM:[173350]

About this Structure

1RJX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Characterization of Lys-698-to-Met substitution in human plasminogen catalytic domain., Terzyan S, Wakeham N, Zhai P, Rodgers K, Zhang XC, Proteins. 2004 Aug 1;56(2):277-84. PMID:15211511

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