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1rkk

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{{STRUCTURE_1rkk| PDB=1rkk | SCENE= }}
{{STRUCTURE_1rkk| PDB=1rkk | SCENE= }}
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'''POLYPHEMUSIN I NMR SOLUTION STRUCTURE'''
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===POLYPHEMUSIN I NMR SOLUTION STRUCTURE===
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==Overview==
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The solution structure of polyphemusin I was determined using (1)H-NMR spectroscopy. Polyphemusin I was found to be an amphipathic, beta-hairpin connected by a type I' beta-turn. The 17 low-energy structures aligned very well over the beta-sheet region while both termini were poorly defined due in part to a hinge-like region centred in the molecule about arginine residues 6 and 16. Conversely, a linear analogue, PM1-S, with all cysteines simultaneously replaced with serine was found to be dynamic in nature, and a lack of medium and long-range NOEs indicated that this molecule displayed no favoured conformation. Circular dichroism (CD) spectroscopy confirmed that in solution, 50% trifluoroethanol (TFE) and in the presence of liposomes, PM1-S remained unstructured. The antimicrobial activity of PM1-S was found to be 4- to 16-fold less than that of polyphemusin I and corresponded with a 4-fold reduction in bacterial membrane depolarization. Both peptides were able to associate with lipid bilayers in a similar fashion; however, PM1-S was completely unable to translocate model membranes while polyphemusin I retained this activity. It was concluded that the disulfide-constrained, beta-sheet structure of polyphemusin I is required for maximum antimicrobial activity. Disruption of this structure results in reduced antimicrobial activity and completely abolishes membrane translocation indicating that the linear PM1-S acts through a different antimicrobial mechanism.
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(as it appears on PubMed at http://www.pubmed.gov), where 15134657 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15134657}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKK OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKK OCA].
==Reference==
==Reference==
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[[Category: Disulfide bridge]]
[[Category: Disulfide bridge]]
[[Category: Polyphemusin]]
[[Category: Polyphemusin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:36:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:52:32 2008''

Revision as of 15:52, 28 July 2008

Image:1rkk.png

Template:STRUCTURE 1rkk

POLYPHEMUSIN I NMR SOLUTION STRUCTURE

Template:ABSTRACT PUBMED 15134657

About this Structure

Full experimental information is available from OCA.

Reference

Structure-activity relationships for the beta-hairpin cationic antimicrobial peptide polyphemusin I., Powers JP, Rozek A, Hancock RE, Biochim Biophys Acta. 2004 May 6;1698(2):239-50. PMID:15134657

Page seeded by OCA on Mon Jul 28 18:52:32 2008

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