7h5h
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of endothiapepsin PF_RT2 in complex with AC40075 at 296 K== | |
| + | <StructureSection load='7h5h' size='340' side='right'caption='[[7h5h]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7h5h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryphonectria_parasitica Cryphonectria parasitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7H5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7H5H FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7h5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7h5h OCA], [https://pdbe.org/7h5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7h5h RCSB], [https://www.ebi.ac.uk/pdbsum/7h5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7h5h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CARP_CRYPA CARP_CRYPA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Advances in structural biology have relied heavily on synchrotron cryo-crystallography and cryogenic electron microscopy to elucidate biological processes and for drug discovery. However, disparities between cryogenic and room-temperature (RT) crystal structures pose challenges. Here, Cryo2RT, a high-throughput RT data-collection method from cryo-cooled crystals that leverages the cryo-crystallography workflow, is introduced. Tested on endothiapepsin crystals with four soaked fragments, thaumatin and SARS-CoV-2 3CL(pro), Cryo2RT reveals unique ligand-binding poses, offers a comparable throughput to cryo-crystallography and eases the exploration of structural dynamics at various temperatures. | ||
| - | + | Cryo2RT: a high-throughput method for room-temperature macromolecular crystallography from cryo-cooled crystals.,Huang CY, Aumonier S, Olieric V, Wang M Acta Crystallogr D Struct Biol. 2024 Aug 1;80(Pt 8):620-628. doi: , 10.1107/S2059798324006697. Epub 2024 Jul 25. PMID:39052318<ref>PMID:39052318</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7h5h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cryphonectria parasitica]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Aumonier S]] | ||
| + | [[Category: Huang C-Y]] | ||
| + | [[Category: Olieric V]] | ||
| + | [[Category: Wang M]] | ||
Current revision
Crystal structure of endothiapepsin PF_RT2 in complex with AC40075 at 296 K
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