1rm9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1rm9.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1rm9.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1rm9| PDB=1rm9 | SCENE= }}
{{STRUCTURE_1rm9| PDB=1rm9 | SCENE= }}
-
'''Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with an Expanded Genetic Code'''
+
===Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with an Expanded Genetic Code===
-
==Overview==
+
<!--
-
The expanded genetic code in combination with site-directed mutagenesis was used to probe spectroscopic and structural roles of tryptophan (Trp) residues in Aequorea victoria green fluorescent proteins (avGFPs). Nine different halogen-, chalcogen-, and methyl-containing Trp isosteric analogues and surrogates were incorporated into avGFPs containing indole moieties in, and outside of, the chromophore, by the use of the selective pressure incorporation method. Such isosteric replacements introduced minimal local geometry changes in indole moieties, often to the level of single atomic exchange ('atomic mutation') and do not affect three-dimensional structures of avGFPs but induce changes in spectral properties. Our approach offers a new platform to re-evaluate issues like resonance transfer, mechanisms of chromophore formation and maturation, as well as the importance of local geometry and weak sulphur-aromatic interactions for avGFP spectral properties and structural stability. The library of novel tailor-made avGFP mutants and variants generated in this work has demonstrated not only the potentials of the expanded genetic code to study spectroscopic functions, but also a new approach to generate tailor-made proteins with interesting and useful spectral properties.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15101562}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15101562 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15101562}}
==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Gfp]]
[[Category: Gfp]]
[[Category: Noncanonical amino acid]]
[[Category: Noncanonical amino acid]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:39:58 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:41:26 2008''

Revision as of 14:41, 28 July 2008

Image:1rm9.png

Template:STRUCTURE 1rm9

Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with an Expanded Genetic Code

Template:ABSTRACT PUBMED 15101562

About this Structure

1RM9 is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Probing the role of tryptophans in Aequorea victoria green fluorescent proteins with an expanded genetic code., Budisa N, Pal PP, Alefelder S, Birle P, Krywcun T, Rubini M, Wenger W, Bae JH, Steiner T, Biol Chem. 2004 Feb;385(2):191-202. PMID:15101562

Page seeded by OCA on Mon Jul 28 17:41:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rm9"
Personal tools