1u76
From Proteopedia
(New page: 200px<br /> <applet load="1u76" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u76, resolution 2.60Å" /> '''Crystal structure o...) |
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| - | [[Image:1u76.gif|left|200px]]<br /> | + | [[Image:1u76.gif|left|200px]]<br /><applet load="1u76" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1u76, resolution 2.60Å" /> | caption="1u76, resolution 2.60Å" /> | ||
'''Crystal structure of hPCNA bound to residues 452-466 of the DNA polymerase-delta-p66 subunit'''<br /> | '''Crystal structure of hPCNA bound to residues 452-466 of the DNA polymerase-delta-p66 subunit'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U76 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1U76 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U76 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sliding clamp]] | [[Category: sliding clamp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:59:24 2008'' |
Revision as of 14:59, 15 February 2008
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Crystal structure of hPCNA bound to residues 452-466 of the DNA polymerase-delta-p66 subunit
Overview
Human Proliferating Cellular Nuclear Antigen (hPCNA), a member of the, sliding clamp family of proteins, makes specific protein-protein, interactions with DNA replication and repair proteins through a small, peptide motif termed the PCNA-interacting protein, or PIP-box. We solved, the structure of hPCNA bound to PIP-box-containing peptides from the p66, subunit of the human replicative DNA polymerase-delta (452-466) at 2.6 A, and of the flap endonuclease (FEN1) (331-350) at 1.85 A resolution. Both, structures demonstrate that the pol-delta p66 and FEN1 peptides interact, with hPCNA at the same site shown to bind the cdk-inhibitor p21(CIP1)., Binding studies indicate that peptides from the p66 subunit of the, pol-delta holoenzyme and FEN1 bind hPCNA from 189- to 725-fold less, tightly than those of p21. Thus, the PIP-box and flanking regions provide, a small docking peptide whose affinities can be readily adjusted in accord, with biological necessity to mediate the binding of DNA replication and, repair proteins to hPCNA.
About this Structure
1U76 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-delta p66 subunit and flap endonuclease-1., Bruning JB, Shamoo Y, Structure. 2004 Dec;12(12):2209-19. PMID:15576034
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