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1u7f
From Proteopedia
(New page: 200px<br /> <applet load="1u7f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u7f, resolution 2.6Å" /> '''Crystal Structure of...) |
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| - | [[Image:1u7f. | + | [[Image:1u7f.jpg|left|200px]]<br /><applet load="1u7f" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1u7f, resolution 2.6Å" /> | caption="1u7f, resolution 2.6Å" /> | ||
'''Crystal Structure of the phosphorylated Smad3/Smad4 heterotrimeric complex'''<br /> | '''Crystal Structure of the phosphorylated Smad3/Smad4 heterotrimeric complex'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U7F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1U7F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tgf-beta]] | [[Category: tgf-beta]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:59:31 2008'' |
Revision as of 14:59, 15 February 2008
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Crystal Structure of the phosphorylated Smad3/Smad4 heterotrimeric complex
Contents |
Overview
The formation of protein complexes between phosphorylated R-Smads and, Smad4 is a central event in the TGF-beta signaling pathway. We have, determined the crystal structure of two R-Smad/Smad4 complexes, Smad3/Smad4 to 2.5 angstroms, and Smad2/Smad4 to 2.7 angstroms. Both, complexes are heterotrimers, comprising two phosphorylated R-Smad subunits, and one Smad4 subunit, a finding that was corroborated by isothermal, titration calorimetry and mutational studies. Preferential formation of, the R-Smad/Smad4 heterotrimer over the R-Smad homotrimer is largely, enthalpy driven, contributed by the unique presence of strong, electrostatic interactions within the heterotrimeric interfaces. The study, supports a common mechanism of Smad protein assembly in TGF-beta, superfamily signaling.
Disease
Known diseases associated with this structure: Juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome OMIM:[600993], Pancreatic cancer OMIM:[600993], Polyposis, juvenile intestinal OMIM:[600993]
About this Structure
1U7F is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of heteromeric smad protein assembly in TGF-beta signaling., Chacko BM, Qin BY, Tiwari A, Shi G, Lam S, Hayward LJ, De Caestecker M, Lin K, Mol Cell. 2004 Sep 10;15(5):813-23. PMID:15350224
Page seeded by OCA on Fri Feb 15 16:59:31 2008
