Pathway of Insulin Secretion through GLP-1 Receptor

Introduction and Function

to signal the body to secrete insulin and inhibit glucagon secretion. When glucose levels in the blood rise (usually after consuming food), GLP-1R activates, creating a signaling cascade to signal cAMP. After the signaling cascade, the final result is the secretion of insulin to the rest of the body. Discovered in 1990s while trying to understand the mechanism of GLP-1 action. [1] The structure was determined using cryogenic electron microscopy.

Disease

Diabetes and Obesity

Nonfucnctional Pathway due to Type II Diabetes

Type II Diabetes is a chronic medical condition in which the pancreas does not properly produce insulin for the body.[2] Due to the lack of insulin in the body, a persons blood glucose levels can be dangerously low. The lack of insulin and low glucose level can be related to the decrease in levels of GLP-1 within the body. When GLP-1 is not bonding properly to its receptor, the insulin regulation in the body is disrupted. When a persons blood sugar levels are too low they can cause a person to become dizzy and tired. There is also the possibility where GLP-1 is not broken down at the proper time which can cause a persons blood sugar to become to high which can lead to a person feeling feverish and sick.

Ligand and Receptor Interactions

Sequence Alignment of GIP, Tirzepatide, and GLP

Hormone Interactions

The start with the hormone docking in the N-terminus before moving the rest of the hormone into place. The ligand uses between two tryptophan residues at the N-terminus to initially dock into the receptor.

Drug Interactions

The drug antagonist is shown in a mint color while the receptor is shown in pink. The interaction between Tirzepetide and the receptor are using of the tryptophan residues. Which is a similar interaction as between the natural hormone and the receptor. The drug antagonist also uses in the N terminus between a tyrosine and glutamate and also tyrosine and glutamine.

Relevance

Tirzepatide and other GLP-1R related antagonist drugs help to regulate blood glucose levels.[3] The GLP-1R antagonists are able to aid the body in proper secretion of insulin because the antagonists are harder for the body to break down. Although these drugs can make remarkable changes, the long term effects of GLP-1R related drugs are unknown. There have been some hints toward thyroid cancers but this was only shown in mice during trial periods.

References

^{[1] [2] [3] [4]}

1. ↑ Coskun T, Sloop KW, Loghin C, Alsina-Fernandez J, Urva S, Bokvist KB, Cui X, Briere DA, Cabrera O, Roell WC, Kuchibhotla U, Moyers JS, Benson CT, Gimeno RE, D'Alessio DA, Haupt A. LY3298176, a novel dual GIP and GLP-1 receptor agonist for the treatment of type 2 diabetes mellitus: From discovery to clinical proof of concept. Mol Metab. 2018 Dec;18:3-14. PMID:30473097 doi:10.1016/j.molmet.2018.09.009
2. ↑ Mayendraraj A, Rosenkilde MM, Gasbjerg LS. GLP-1 and GIP receptor signaling in beta cells interactions and co-stimulation. Peptides. 2022 May;151:170749. PMID:35065096 doi:10.1016/j.peptides.2022.170749
3. ↑ Seino Y, Fukushima M, Yabe D. GIP and GLP-1, the two incretin hormones: Similarities and differences. J Diabetes Investig. 2010 Apr 22;1(1-2):8-23. PMID:24843404 doi:10.1111/j.2040-1124.2010.00022.x
4. ↑ Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW. Structural determinants of dual incretin receptor agonism by tirzepatide. Proc Natl Acad Sci U S A. 2022 Mar 29;119(13):e2116506119. PMID:35333651 doi:10.1073/pnas.2116506119