This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1rtc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1rtc.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1rtc.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1rtc| PDB=1rtc | SCENE= }}
{{STRUCTURE_1rtc| PDB=1rtc | SCENE= }}
-
'''THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS'''
+
===THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS===
-
==Overview==
+
<!--
-
The plant cytotoxin ricin is a heterodimer with a cell surface binding (B) chain and an enzymatically active A chain (RTA) known to act as a specific N-glycosidase. RTA must be separated from B chain to attack rRNA. The X-ray structure of ricin has been solved recently; here we report the structure of the isolated A chain expressed from a clone in Escherichia coli. This structure of wild-type rRTA has and will continue to serve as the parent compound for difference Fouriers used to assess the structure of site-directed mutants designed to analyze the mechanism of this medically and commercially important toxin. The structure of the recombinant protein, rRTA, is virtually identical to that seen previously for A chain in the heterodimeric toxin. Some minor conformational changes due to interactions with B chain and to crystal packing differences are described. Perhaps the most significant difference is the presence in rRTA of an additional active site water. This molecule is positioned to act as the ultimate nucleophile in the depurination reaction mechanism proposed by Monzingo and Robertus (1992, J. Mol. Biol. 227, 1136-1145).
+
The line below this paragraph, {{ABSTRACT_PUBMED_8453380}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 8453380 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_8453380}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Robertus, J D.]]
[[Category: Robertus, J D.]]
[[Category: Glycosidase]]
[[Category: Glycosidase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:53:00 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:19:36 2008''

Revision as of 01:19, 28 July 2008

Image:1rtc.png

Template:STRUCTURE 1rtc

THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS

Template:ABSTRACT PUBMED 8453380

About this Structure

1RTC is a Single protein structure of sequence from Ricinus communis. Full crystallographic information is available from OCA.

Reference

Structure of recombinant ricin A chain at 2.3 A., Mlsna D, Monzingo AF, Katzin BJ, Ernst S, Robertus JD, Protein Sci. 1993 Mar;2(3):429-35. PMID:8453380

Page seeded by OCA on Mon Jul 28 04:19:36 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rtc"
Personal tools