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1rxm

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{{STRUCTURE_1rxm| PDB=1rxm | SCENE= }}
{{STRUCTURE_1rxm| PDB=1rxm | SCENE= }}
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'''C-terminal region of FEN-1 bound to A. fulgidus PCNA'''
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===C-terminal region of FEN-1 bound to A. fulgidus PCNA===
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==Overview==
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Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
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(as it appears on PubMed at http://www.pubmed.gov), where 14718165 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14718165}}
==About this Structure==
==About this Structure==
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[[Category: Sliding clamp]]
[[Category: Sliding clamp]]
[[Category: Torus]]
[[Category: Torus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:01:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:34:38 2008''

Revision as of 06:34, 28 July 2008

Image:1rxm.png

Template:STRUCTURE 1rxm

C-terminal region of FEN-1 bound to A. fulgidus PCNA

Template:ABSTRACT PUBMED 14718165

About this Structure

1RXM is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:14718165

Page seeded by OCA on Mon Jul 28 09:34:38 2008

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