1rxv

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{{STRUCTURE_1rxv| PDB=1rxv | SCENE= }}
{{STRUCTURE_1rxv| PDB=1rxv | SCENE= }}
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'''Crystal Structure of A. Fulgidus FEN-1 bound to DNA'''
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===Crystal Structure of A. Fulgidus FEN-1 bound to DNA===
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==Overview==
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Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
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{{ABSTRACT_PUBMED_14718165}}
==About this Structure==
==About this Structure==
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[[Category: Helix-3 turn-helix]]
[[Category: Helix-3 turn-helix]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:02:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:38:23 2008''

Revision as of 19:38, 28 July 2008

Image:1rxv.png

Template:STRUCTURE 1rxv

Crystal Structure of A. Fulgidus FEN-1 bound to DNA

Template:ABSTRACT PUBMED 14718165

About this Structure

1RXV is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:14718165

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