1s2h

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[[Image:1s2h.gif|left|200px]]
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{{Seed}}
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[[Image:1s2h.png|left|200px]]
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{{STRUCTURE_1s2h| PDB=1s2h | SCENE= }}
{{STRUCTURE_1s2h| PDB=1s2h | SCENE= }}
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'''The Mad2 spindle checkpoint protein possesses two distinct natively folded states'''
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===The Mad2 spindle checkpoint protein possesses two distinct natively folded states===
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==Overview==
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The spindle checkpoint delays chromosome segregation in response to misaligned sister chromatids during mitosis, thus ensuring the fidelity of chromosome inheritance. Through binding to Cdc20, the Mad2 spindle checkpoint protein inhibits the target of this checkpoint, the ubiquitin protein ligase APC/C(Cdc20). We now show that without cofactor binding or covalent modification Mad2 adopts two distinct folded conformations at equilibrium (termed N1-Mad2 and N2-Mad2). The structure of N2-Mad2 has been determined by NMR spectroscopy. N2-Mad2 is much more potent in APC/C inhibition. Overexpression of a Mad2 mutant that specifically sequesters N2-Mad2 partially blocks checkpoint signaling in living cells. The two Mad2 conformers interconvert slowly in vitro, but interconversion is accelerated by a fragment of Mad1, an upstream regulator of Mad2. Our results suggest that the unusual two-state behavior of Mad2 is critical for spindle checkpoint signaling.
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The line below this paragraph, {{ABSTRACT_PUBMED_15024386}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15024386 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15024386}}
==About this Structure==
==About this Structure==
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1S2H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2H OCA].
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1S2H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2H OCA].
==Reference==
==Reference==
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[[Category: Mad2]]
[[Category: Mad2]]
[[Category: Spindle checkpoint protein]]
[[Category: Spindle checkpoint protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:12:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:41:41 2008''

Revision as of 05:41, 29 July 2008

Image:1s2h.png

Template:STRUCTURE 1s2h

The Mad2 spindle checkpoint protein possesses two distinct natively folded states

Template:ABSTRACT PUBMED 15024386

About this Structure

1S2H is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

The Mad2 spindle checkpoint protein has two distinct natively folded states., Luo X, Tang Z, Xia G, Wassmann K, Matsumoto T, Rizo J, Yu H, Nat Struct Mol Biol. 2004 Apr;11(4):338-45. Epub 2004 Mar 14. PMID:15024386

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