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| - | [[Image:1s6d.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1s6d| PDB=1s6d | SCENE= }} | | {{STRUCTURE_1s6d| PDB=1s6d | SCENE= }} |
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| - | '''Structure in solution of a methionine-rich 2S Albumin protein from Sunflower Seed'''
| + | ===Structure in solution of a methionine-rich 2S Albumin protein from Sunflower Seed=== |
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| - | ==Overview==
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| - | The three-dimensional structure in aqueous solution of SFA-8, a 2S albumin 103-residue protein from seeds of sunflower (Helianthus anuus L.), has been determined by NMR methods. An almost complete (1)H resonance assignment was accomplished from analysis of two-dimensional (2D) COSY and 2D TOCSY spectra, and the structure was computed by using restrained molecular dynamics on the basis of 1393 upper limit distance constraints derived from NOE cross-correlation intensities measured in 2D NOESY spectra. In contrast with most other 2S albumins, SFA-8 consists of a single polypeptide chain without any cleavage in the segment of residues 30-46. The computed structures exhibited an rmsd radius of 0.52 A for the backbone structural core (residues 11-30 and 46-101) and 1.01 A for the side chain heavy atoms. The resulting structure consists of five amphipathic helices arranged in a right-handed superhelix, a folding motif first observed in nonspecific lipid transfer (nsLTP) proteins, and common to other 2S albumins. In contrast to nsLTP proteins, neither SFA-8 nor RicC3 (a 2S albumin from castor bean) has an internal cavity that is able to host a lipid molecule, which results from an exchange in the pairing of disulfide bridges in the CXC segment. Both 2S albumins and nonspecific lipid transfer proteins belong to the prolamin superfamily, which includes a number of important food allergens. Differences in the extension and solvent exposition of the so-called "hypervariable loop" (which connects helices III and IV) in SFA-8 and RicC3 may be responsible for the different allergenic properties of the two proteins. SFA-8 has been shown to form highly stable emulsions with oil/water mixtures. We propose that these properties may be determined partly by a hydrophobic patch at the surface of the protein which consists of five methionines that partially hide the Trp76 residue. The flexibility of the loop which contains Trp76 and the hydrophobicity of the whole environment may favor a conformational change, by which the Trp76 side chain may become inserted into the oil phase. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15170335}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15170335 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15170335}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1S6D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helianthus_annuus Helianthus annuus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6D OCA]. | + | 1S6D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helianthus_annuus Helianthus annuus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6D OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Folded leaf]] | | [[Category: Folded leaf]] |
| | [[Category: Right-handed superhelix]] | | [[Category: Right-handed superhelix]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:21:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:08:26 2008'' |
Revision as of 16:08, 28 July 2008
Template:STRUCTURE 1s6d
Structure in solution of a methionine-rich 2S Albumin protein from Sunflower Seed
Template:ABSTRACT PUBMED 15170335
About this Structure
1S6D is a Single protein structure of sequence from Helianthus annuus. Full experimental information is available from OCA.
Reference
Solution structure of a methionine-rich 2S albumin from sunflower seeds: relationship to its allergenic and emulsifying properties., Pantoja-Uceda D, Shewry PR, Bruix M, Tatham AS, Santoro J, Rico M, Biochemistry. 2004 Jun 8;43(22):6976-86. PMID:15170335
Page seeded by OCA on Mon Jul 28 19:08:26 2008
