1s6j

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{{STRUCTURE_1s6j| PDB=1s6j | SCENE= }}
{{STRUCTURE_1s6j| PDB=1s6j | SCENE= }}
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'''N-terminal Region of the Ca2+-saturated calcium regulatory domain (CLD) from Soybean Calcium-dependent Protein Kinase-alpha (CDPK)'''
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===N-terminal Region of the Ca2+-saturated calcium regulatory domain (CLD) from Soybean Calcium-dependent Protein Kinase-alpha (CDPK)===
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==Overview==
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Ca(2+)-dependent protein kinases (CDPKs) are vital Ca(2+)-signaling proteins in plants and protists which have both a kinase domain and a self-contained calcium regulatory calmodulin-like domain (CLD). Despite being very similar to CaM (&gt;40% identity) and sharing the same fold, recent biochemical and structural evidence suggests that the behavior of CLD is distinct from its namesake, calmodulin. In this study, NMR spectroscopy is employed to examine the structure and backbone dynamics of a 168 amino acid Ca(2+)-saturated construct of the CLD (NtH-CLD) in which almost the entire C-terminal domain is exchange broadened and not visible in the NMR spectra. Structural characterization of the N-terminal domain indicates that the first Ca(2+)-binding loop is significantly more open than in a recently reported structure of the CLD complexed with a putative intramolecular binding region (JD) in the CDPK. Backbone dynamics suggest that parts of the third helix exhibit unusually high mobility, and significant exchange, consistent with previous findings that this helix interacts with the C-terminal domain. Dynamics data also show that the "tether" region, consisting of the first 11 amino acids of CLD, is highly mobile and these residues exhibit distinctive beta-type secondary structure, which may help to position the JD and CLD. Finally, the unusual global dynamic behavior of the protein is rationalized on the basis of possible interdomain rearrangements and the highly variable environments of the C- and N-terminal domains.
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(as it appears on PubMed at http://www.pubmed.gov), where 15568805 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15568805}}
==About this Structure==
==About this Structure==
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1S6J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6J OCA].
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1S6J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6J OCA].
==Reference==
==Reference==
Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15568805 15568805]
Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15568805 15568805]
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Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha., Weljie AM, Vogel HJ, J Biol Chem. 2004 Aug 20;279(34):35494-502. Epub 2004 May 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15155727 15155727]
[[Category: Glycine max]]
[[Category: Glycine max]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Ef-hand]]
[[Category: Ef-hand]]
[[Category: Helix-loop-helix]]
[[Category: Helix-loop-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:21:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:34:07 2008''

Revision as of 00:34, 29 July 2008

Image:1s6j.png

Template:STRUCTURE 1s6j

N-terminal Region of the Ca2+-saturated calcium regulatory domain (CLD) from Soybean Calcium-dependent Protein Kinase-alpha (CDPK)

Template:ABSTRACT PUBMED 15568805

About this Structure

1S6J is a Single protein structure of sequence from Glycine max. Full experimental information is available from OCA.

Reference

Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:15568805

Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha., Weljie AM, Vogel HJ, J Biol Chem. 2004 Aug 20;279(34):35494-502. Epub 2004 May 20. PMID:15155727

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