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1s6x

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{{STRUCTURE_1s6x| PDB=1s6x | SCENE= }}
{{STRUCTURE_1s6x| PDB=1s6x | SCENE= }}
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'''Solution structure of VSTx'''
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===Solution structure of VSTx===
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==Overview==
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VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and SGTx, two toxins that inhibit eukaryotic voltage-activated K(+) channels by binding to voltage sensors. VSTx1 has been recently shown to interact equally well with lipid membranes that contain zwitterionic or acidic phospholipids, and it has been proposed that the toxin receptor is located within a region of the channel that is submerged in the membrane. As a first step toward understanding the inhibitory mechanism of VSTx1, we determined the three-dimensional solution structure of the toxin using NMR. Although the structure of VSTx1 is similar to HaTx and SGTx in terms of molecular fold and amphipathic character, the detailed positions of hydrophobic and surrounding charged residues in VSTx1 are very different than what is seen in the other toxins. The amphipathic character of VSTx1, notably the close apposition of basic and hydrophobic residues on one face of the toxin, raises the possibility that the toxin interacts with interfacial regions of the membrane. We reinvestigated the partitioning of VSTx1 into lipid membranes and find that VSTx1 partitioning requires negatively charged phospholipids. Intrinsic tryptophan fluorescence and acrylamide quenching experiments suggest that tryptophan residues on the hydrophobic surface of VSTx1 have a diminished exposure to water when the toxin interacts with membranes. The present results suggest that if membrane partitioning is involved in the mechanism by which VSTx1 inhibits voltage-activated K(+) channels, then binding of the toxin to the channel would likely occur at the interface between the polar headgroups and the hydrophobic phase of the membrane.
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The line below this paragraph, {{ABSTRACT_PUBMED_15835890}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15835890 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15835890}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6X OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6X OCA].
==Reference==
==Reference==
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[[Category: Double stranded antiparallel beta-sheet]]
[[Category: Double stranded antiparallel beta-sheet]]
[[Category: Inhibitory cystine knot]]
[[Category: Inhibitory cystine knot]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:22:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:00:06 2008''

Revision as of 06:00, 29 July 2008

Image:1s6x.png

Template:STRUCTURE 1s6x

Solution structure of VSTx

Template:ABSTRACT PUBMED 15835890

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel., Jung HJ, Lee JY, Kim SH, Eu YJ, Shin SY, Milescu M, Swartz KJ, Kim JI, Biochemistry. 2005 Apr 26;44(16):6015-23. PMID:15835890

Page seeded by OCA on Tue Jul 29 09:00:06 2008

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