1s8e

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{{STRUCTURE_1s8e| PDB=1s8e | SCENE= }}
{{STRUCTURE_1s8e| PDB=1s8e | SCENE= }}
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'''Crystal structure of Mre11-3'''
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===Crystal structure of Mre11-3===
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==Overview==
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The Mre11, Rad50 and Nbs1 proteins make up the conserved multi-functional Mre11 (MRN) complex involved in multiple, critical DNA metabolic processes including double-strand break repair and telomere maintenance. The Mre11 protein is a nuclease with broad substrate recognition, but MRN-dependent processes requiring the nuclease activity are not clearly defined. Here, we report the functional and structural characterization of a nuclease-deficient Mre11 protein termed mre11-3. Importantly, the hmre11-3 protein has wild-type ability to bind DNA, Rad50 and Nbs1; however, nuclease activity was completely abrogated. When expressed in cell lines from patients with ataxia telangiectasia-like disorder (ATLD), hmre11-3 restored the formation of ionizing radiation-induced foci. Consistent with the biochemical results, the 2.3 A crystal structure of mre11-3 from Pyrococcus furiosus revealed an active site structure with a wild-type-like metal-binding environment. The structural analysis of the H85L mutation provides a detailed molecular basis for the ability of mre11-3 to bind but not hydrolyze DNA. Together, these results establish that the mre11-3 protein provides an excellent system for dissecting nuclease-dependent and independent functions of the Mre11 complex.
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{{ABSTRACT_PUBMED_15047855}}
==About this Structure==
==About this Structure==
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[[Category: Mre11]]
[[Category: Mre11]]
[[Category: Rad50]]
[[Category: Rad50]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:25:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:00:31 2008''

Revision as of 09:00, 29 July 2008

Image:1s8e.png

Template:STRUCTURE 1s8e

Crystal structure of Mre11-3

Template:ABSTRACT PUBMED 15047855

About this Structure

1S8E is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of Mre11-3., Arthur LM, Gustausson K, Hopfner KP, Carson CT, Stracker TH, Karcher A, Felton D, Weitzman MD, Tainer J, Carney JP, Nucleic Acids Res. 2004 Mar 26;32(6):1886-93. Print 2004. PMID:15047855

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