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1s94

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[[Image:1s94.gif|left|200px]]
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{{STRUCTURE_1s94| PDB=1s94 | SCENE= }}
{{STRUCTURE_1s94| PDB=1s94 | SCENE= }}
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'''Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei'''
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===Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei===
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==Overview==
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BACKGROUND: Intracellular membrane fusion processes are mediated by the spatial and temporal control of SNARE complex assembly that results in the formation of a four-helical bundle, composed of one vesicle SNARE and three target membrane SNARE polypeptide chains. Syntaxins are essential t-SNAREs and are characterized by an N-terminal Habc domain, a flexible linker region, a coiled-coil or SNARE motif and a membrane anchor. The N-terminal Habc domain fulfills important regulatory functions while the coiled-coil motif, present in all SNAREs, is sufficient for SNARE complex formation, which is thought to drive membrane fusion. RESULTS: Here we report the crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei, s-syntaxin. Squid Habc crystallizes as a dimer and the monomer structure consists of a three-helical bundle. One molecule is strikingly similar to mammalian syntaxin 1A while the second one shows a structural deviation from the common fold in that the C-terminal part of helix C unwinds and adopts an extended conformation. CONCLUSION: Conservation of surface residues indicates that the cytosolic part of s-syntaxin can adopt an auto-inhibitory closed conformation that may bind squid neuronal Sec1, s-Sec1, in the same manner as observed in structure of the rat nSec1/syntaxin 1A complex. Furthermore, despite the overall structural similarity, the observed changes at the C-terminus of one molecule indicate structural plasticity in neuronal syntaxin. Implications of the structural conservation and the changes are discussed with respect to potential Habc domain binding partners such as Munc13, which facilitates the transition from the closed to the open conformation.
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(as it appears on PubMed at http://www.pubmed.gov), where 15113421 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15113421}}
==About this Structure==
==About this Structure==
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[[Category: Structural plasticity]]
[[Category: Structural plasticity]]
[[Category: Three helix bundle]]
[[Category: Three helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:26:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:16:06 2008''

Revision as of 05:16, 28 July 2008

Image:1s94.png

Template:STRUCTURE 1s94

Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei

Template:ABSTRACT PUBMED 15113421

About this Structure

1S94 is a Single protein structure of sequence from Loligo pealei. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei reveals conformational plasticity at its C-terminus., Bracher A, Weissenhorn W, BMC Struct Biol. 2004 Mar 15;4:6. PMID:15113421

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