1s9r

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{{STRUCTURE_1s9r| PDB=1s9r | SCENE= }}
{{STRUCTURE_1s9r| PDB=1s9r | SCENE= }}
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'''CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE'''
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===CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE===
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==Overview==
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Arginine deiminase (ADI), an enzyme that hydrolyzes arginine to generate energy in many parasitic microorganisms, has potent anticancer activities and can halt growth of solid tumors. We determined the crystal structure of ADI from Mycoplasma arginini in two different forms (1.6 and 2.0 A resolution) using multiple isomorphous replacement. ADI shares common structural features with the arginine-catabolizing enzymes Arg:Gly amidinotransferase and dimethylarginine dimethyl-aminohydrolase; ADI contains an additional domain of five helices. The scissile C-N bonds of the substrates and the catalytic triads (Cys398-His269-Glu213 of ADI) for the three enzymes superimpose on each other. The ADI structure from form I crystals corresponds to a tetrahedral intermediate with four heteroatoms (1S, 2N, 1O) covalently bonded to the reaction-center carbon. The structure from form II crystals represents an amidino-enzyme complex; the reaction-center carbon is covalently bonded to Cys398 sulfur and two nitrogens, and the reacting water molecule is only 2.54 A away.
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(as it appears on PubMed at http://www.pubmed.gov), where 15062088 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15062088}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Raction intermediate]]
[[Category: Raction intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:27:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:27:54 2008''

Revision as of 03:28, 29 July 2008

Image:1s9r.png

Template:STRUCTURE 1s9r

CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE

Template:ABSTRACT PUBMED 15062088

About this Structure

1S9R is a Single protein structure of sequence from Mycoplasma arginini. Full crystallographic information is available from OCA.

Reference

Crystal structures of arginine deiminase with covalent reaction intermediates; implications for catalytic mechanism., Das K, Butler GH, Kwiatkowski V, Clark AD Jr, Yadav P, Arnold E, Structure. 2004 Apr;12(4):657-67. PMID:15062088

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