Journal:Acta Cryst F:S2053230X24004655
From Proteopedia
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<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| - | RSF complex belongs to ISWI chromatin remodeling family and is composed of two subunits which are RSF1 ( | + | RSF complex belongs to ISWI chromatin remodeling family and is composed of two subunits which are RSF1 ('''R'''emodeling and '''S'''pacing '''F'''actor '''1''') and SNF2h ('''S'''ucrose '''N'''on'''F'''ermenting protein '''2''' Homolog). RSF complex participates in nucleosome spacing and assembly and then promotes nucleosome maturation. Though SNF2h has been studied extensively in the last few years, the remodeler RSF’s structural and functional properties still remain vague. Here, we report the cryoEM structure of RSF-nucleosome complex, the 3D model shows a two-lobe architecture of RSF, and the RSF-nucleosome (flanking with linker DNA) complex structure shows RSF complex moves the DNA away from the histone octamer surface at the DNA entry point. Additionally, nucleosome sliding assay and restriction enzyme accessibility assays (REAA) show that RSF1 subunit may bring changes in the SNF2h chromatin remodeling properties. As a “nucleosome ruler”，our RSF-dinucleosomes binding affinity test results propose that the critical distance RSF “measures” between two nucleosomes is about 24 bp. |
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 07:41, 23 May 2024
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This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
