1sf1

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[[Image:1sf1.jpg|left|200px]]
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{{Seed}}
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{{STRUCTURE_1sf1| PDB=1sf1 | SCENE= }}
{{STRUCTURE_1sf1| PDB=1sf1 | SCENE= }}
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'''NMR STRUCTURE OF HUMAN INSULIN under Amyloidogenic Condition, 15 STRUCTURES'''
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===NMR STRUCTURE OF HUMAN INSULIN under Amyloidogenic Condition, 15 STRUCTURES===
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==Overview==
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Insulin undergoes aggregation-coupled misfolding to form a cross-beta assembly. Such fibrillation has long complicated its manufacture and use in the therapy of diabetes mellitus. Of interest as a model for disease-associated amyloids, insulin fibrillation is proposed to occur via partial unfolding of a monomeric intermediate. Here, we describe the solution structure of human insulin under amyloidogenic conditions (pH 2.4 and 60 degrees C). Use of an enhanced sensitivity cryogenic probe at high magnetic field avoids onset of fibrillation during spectral acquisition. A novel partial fold is observed in which the N-terminal segments of the A- and B-chains detach from the core. Unfolding of the N-terminal alpha-helix of the A-chain exposes a hydrophobic surface formed by native-like packing of the remaining alpha-helices. The C-terminal segment of the B-chain, although not well ordered, remains tethered to this partial helical core. We propose that detachment of N-terminal segments makes possible aberrant protein-protein interactions in an amyloidogenic nucleus. Non-cooperative unfolding of the N-terminal A-chain alpha-helix resembles that observed in models of proinsulin folding intermediates and foreshadows the extensive alpha --&gt; beta transition characteristic of mature fibrils.
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The line below this paragraph, {{ABSTRACT_PUBMED_14988398}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14988398 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14988398}}
==Disease==
==Disease==
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==About this Structure==
==About this Structure==
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1SF1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SF1 OCA].
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1SF1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SF1 OCA].
==Reference==
==Reference==
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[[Category: Hormone]]
[[Category: Hormone]]
[[Category: Human insulin]]
[[Category: Human insulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:37:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:58:37 2008''

Revision as of 13:58, 28 July 2008

Image:1sf1.png

Template:STRUCTURE 1sf1

Contents

NMR STRUCTURE OF HUMAN INSULIN under Amyloidogenic Condition, 15 STRUCTURES

Template:ABSTRACT PUBMED 14988398

Disease

Known disease associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]

About this Structure

1SF1 is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

Mechanism of insulin fibrillation: the structure of insulin under amyloidogenic conditions resembles a protein-folding intermediate., Hua QX, Weiss MA, J Biol Chem. 2004 May 14;279(20):21449-60. Epub 2004 Feb 26. PMID:14988398

Page seeded by OCA on Mon Jul 28 16:58:37 2008

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